摘要
目的研究热量限制对老年早期大鼠的生存状态和行为学的影响。方法选用18月龄的健康雄性SD大鼠，根据进食情况分为2组，一组为CR组（n=29）给予对照组进食量60％的食量饲养，对照组为ad libitum（AL）组（n=31）自由进食，观察6个月，在此期间，记录大鼠的生存状况，每周测定大鼠体质量；6个月后，进行旷场实验观察自发活动能力。结果CR组给予60％的饮食控制后，体质量维持在低于对照组约20％左右（P〈0．01）。CR组的生存率为89．66％显著高于AL组67．74％（P=0．039）；在旷场实验中测定的CR组大鼠自发活动路程为（201．06±78．35）m，显著长于AL组[（147．38±36．89）m，P=0．021]，而CR组的自发活动速度为（78．10±34．38）mm／s也显著高于AL组[（48．96±14．87）mm／s，P=0．021]，并且CR组大鼠具有在中央区域的活动距离长（P=0．048）和在周边区域活动速度快（P=0．012）的特点。结论对老年早期的大鼠给予6个月的热量限制可以提高大鼠的生存率以及旷场活动能力，反映了大鼠的健康状况改善。
Objective To investigate the effect of a shorter period of CR from early old age rats on the survival and health status by behavioral study. Methods Male SD rats with age of 18 months were subjected to restricted intake in CR group （n = 29） by 60% comparing with that of rats fed ad libitum （AL） （n = 31 ） for 6 months. The overall health status, including survival and locomotor activity by open-field test was compared. Resuits Growth rate of the CR rats demonstrated a period of decline during the first 10 weeks of treatment, and thereafter became steady below that of AL rats （P〈 0.01 ）. The CR rats showed a higher survival 89.66% than AL rats 67.74% （P = 0. 039）. In the open-field test, the CR rats traveled longer [ （ CR ： 201.06 ± 78.35 ） m, AL ： （ 147.38 ± 36.89） m, P = 0.021 ] especially at the centre place （P = 0.048）, and moved faster [ （ CR ：78.10 ± 34.38） mm/s, AL： （48.96 ± 14.87） mm/s, P=0.021 ] as well as in circumjacent area （P=0. 012） than the AL rats. Conclusion The 6-month treatment of 60% CR elevated survival of early old age rat. The 6-month treatment of 60% CR benefited health status by locomotor activity.
出处
《中国行为医学科学》
CSCD
2008年第12期1060-1061,共2页
Chinese Journal of Behavioral Medical Science
基金
广东省卫生厅科研基金（B2006103）
汕头市重点科技计划项目
关键词
热量限制
生存率
自发活动
Caloric restriction
Survival
Locomotor activity
