摘要
目的观察大鼠去势后体温改变与下丘脑视前区5-羟色胺2A(5-HT2A)受体亚型表达之间的关系。方法用数字温度计TM902C检测大鼠的肛温,酶联免疫吸附试验测定血清激素水平,根据5-HT2A受体互补DNA序列合成相应的特异性引物,用PCR法观察大鼠去卵巢后下丘脑视前区5-HT2A受体表达。结果①大鼠去势组(OVX)自第八周末肛温明显高于假去势(sham)组(P<0.05)。②OVX组大鼠血清17β-雌二醇(17β-estradiol,E2)较sham组下降,LH水平升高,差异显著(P<0.05)。③OVX组下丘脑视前区5-HT2A受体表达增强,与sham组比较有显著性差异(P<0.05)。结论大鼠卵巢摘除后体温升高,下丘脑视前区5-HT2A受体表达增强,提示5-HT在低雌激素状态下的体温升高调节中起一定作用。
Objective To observe the relationship between temperature change and the expression of 5 HT2A recep tor subtype in preoptic area and anterior hypothalamus of ovarieetomized（OVX） rats. Methods The digital thermometer TM902C was used to measure rectal temperature （RT）. Enzyme-linked immunoadsordent assay （ELISA） was used to detect serum hormone levels. The method of polymerase chain reaction （PCR） was used to detect the presence of 5-HT2Areceptor subtype in the preoptic area and anterior hypothalamus of rat after ovariectomy. The specific olig-onucleotide primers were synthesized based on the complementary DNA sequences for the rat 5-HT2Areceptor subtype. Results ①The RT in OVX rats was significantly elevated 8 weeks after ovariectomy compared with those in sharn operated control group （P〈0. 05）. ②Compared with the control group, concentrations of serum E2 decreased in the OVX group （P〈0. 05）, while concentration of LH inereased （P〈0. 05）. ③The expression of 5-HT2A receptor subtype in preoptic area and anterior hypothalamus of the OVX rats was significantly higher than control group. Conclusion The RT was elevated after ovariectomy and the expression of 5-HT2A receptor subtype in preoptic area and anterior hypothalamus of the OVX rats were significantly higher. Serotonin may play some roles in thermoregulation in the state of low hormone eoneentrations.
出处
《华南国防医学杂志》
CAS
2008年第6期25-28,共4页
Military Medical Journal of South China
