摘要
研究交感神经阻断对辐照小鼠的T细胞、T细胞亚群的影响。小鼠受0.5Gy、3Gy、6Gy辐照后,6-羟多巴胺阻断交感神经。阻断两周后小鼠眼眶处取血,利用流式细胞仪检测小鼠外周血中T细胞、T细胞亚群的数量以及它们活化细胞数量。交感神经阻断对受辐照小鼠胸腺指数具有极为显著的降低作用。与正常对照组相比,受辐照小鼠的T、Th和Tc/Ts细胞数量均随辐照剂量的增加而减少,在相同辐照剂量下,虽然阻断组的T细胞数量小于未阻断组,但是Th和Tc/Ts细胞数量均明显大于未阻断组。与正常对照组相比,受辐照小鼠的CD3+CD25+、活化Th细胞和活化Tc/Ts细胞数量随辐照剂量的增大反而有所提高,并且在相同的辐照剂量下阻断组的CD3+CD25+、活化Th细胞和活化Tc/Ts细胞数量均明显大于非阻断组。表明交感神经阻断进一步增强了受辐照机体的免疫抑制作用。
In this paper the effects of sympathectomy on T lymphocytes of irradiated mice were investigated. The whole blood sample was collected from mice irradiated with 0.5Gy, 3Gy and 6Gy and injected with 6-OHDA respectively. The number of T lymphocytes, T lymphocyte subsets and their activated cells were detected by flow cytometry. Results show that the thymus index of irradiated mice with sympathectomy obviously decline. And the number of T, Th and Tc/Ts cells collected from irradiated mice decrease with the increment of absorbed doses. The number of Th, Tc/Ts cells becomes obviously larger though the number of T cells of sympathectomized group is smaller than that of the group without sympathectomy at the same absorbed doses. Compared with the control group, the number of CD3^＋ CD25^＋, activated Th and Tc/Ts cells increase with the increment of absorbed doses while the parameter of the sympathectomized sample are larger than that of control group. All these indicate that the sympathetic nerve has a positive function to the immune function of irradiated mice.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
2008年第6期348-352,共5页
Journal of Radiation Research and Radiation Processing
