摘要
目的研究抑癌基因P16和血管内皮生长因子(VEGF)在胃癌组织中的表达及与临床病理学参数之间的关系。方法采用链霉素抗生物素蛋白-过氧化物酶(streptaridin peroxidase,SP)免疫组织化学方法及形态定量分析方法,检测胃癌78例、胃良性病变15例组织中P16蛋白及VEGF的表达情况。结果胃癌标本VEGF阳性表达率高于胃良性病变,VEGF主要表达于肿瘤细胞胞浆或胞膜,与胃癌淋巴结转移、临床分期有关(P<0.01);胃癌中的P16蛋白阳性表达率低于胃良性病变,P16蛋白主要表达于肿瘤细胞胞核,其表达与胃癌淋巴结转移、临床分期有关(P<0.05);胃癌中P16蛋白的低表达与VEGF的高表达具有显著相关性(P<0.01)。结论VEGF促进肿瘤血管生成,VEGF表达上调或P16蛋白表达下调对胃癌发生、发展及转移起重要作用,为判定肿瘤的生物学行为和转移潜能提供了重要指标。
Objective To research the behaviour of P16-cancer suppressor gene and Vascular endothelial growth factor （VEGF） in Gastric cancer tissue and the relationship between it and Clinical pathology parameters. Methods The expression of P16 and VEGF in 78 specimens of gastric carcinoma and 15 specimens of benign lesion of stomach were detected by S-P immunohistochemieal method and the morphometry quantitative method. Results The positive expression rate of VEGF in gastric carcinoma was taller than in benign lesion of stomach and VEGF main express in tumour cytoplasm or membrane, and it related with lymph node metastasis of gastric cancer and clinical stage（ P 〈 0.01） .The positive expression rate of P16 in gastric carcinoma was lower than in benign lesion of stomach and P16 main express in turnout nuclear, and it related with lymph node metastasis of gastric cancer and clinical stage（ P 〈 0.05）. P16 high expression notability related with VEGF low expression（ P 〈 0.01 ）. Conduslon VEGF promote tumor angiogenesis. Up regulation of VEGF expression or down regulation of P16 expression is very important role to the occurrence, development and transfer of gastric carcinoma, and it provide a very important indicator for biological behavior and metastasis in patients of tumour.
出处
《中国实验诊断学》
2008年第12期1543-1545,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技发展计划(20060908-02)资助项目
