摘要
目的:筛选小鼠肝损伤敏感基因,用于中药及其它外源物肝脏毒性的快速评价。方法:分别研究小鼠给予酒精、CCl4、卡介苗+脂多糖、黄药子和千里光乙醇提取物后肝组织基因表达谱,筛选与肝损伤密切相关的共同差异表达基因。结果:从上述5个给药组中筛选到7条功能已知基因(BC05200、NM-019410、NM-173019、AB028272、AK088925、AK030862和AK088816)和1条功能未知基因(BC069871)。除AK088816差异上调表达外,其他7条基因均差异下调表达。结论:这些共同差异表达基因主要参与了肝细胞的糖代谢、细胞凋亡、细胞生长周期、细胞骨架与信号传导、蛋白质折叠与泛素化等生物学过程,与肝损伤关系十分密切。
Objective： To scan the common differentially expressed genes in mice liver tissue following subacute exposure to bepatotoxicants, including alcohol, carbon tetrachloride, BCG ＆ LPS, the alcohol extracts of Rhizoma Dioscoreae Bulbiferae and Senecio scandens Buch.-Ham., and apply to the rapid toxic evaluation of TCM and other xenobiotics. Methods：The gene expression profiles of mice liver tissue, after respective administration of alcohol, CCl4, BCG associated with LPS, the alcohol extracts of Rhizoma Dioscoreae Bulbiferae and Senecio scandens Buch.-Ham. to healthy mice, were analyzed by use of mouse genome cDNA microarray. The correlations between the common differential expressed genes and the liver injury were analyzed based on the biological functions of those differentially expressed genes. Results： Among all of the five drug administration groups, there were 7 known function genes（BC05200, NM-019410,NM-173019, AB028272, AK088925, AK030862 and AK088816）and 1 unknown function gene（BC069871）differentially expressed. Beside AK088816, the other 7 genes were all down-regulate expressed. Conclusion： The common differentially expressed genes participate in the process of saccharometabolism, apoptosis, cell growth cycle, cytoskeleton and signal transmission, protein folding and protein ubiquitin. The abnormal expression of the common genes were closely correlated to the liver, and might be important for rapid hepatoxicity elucidation of traditional Chinese medicine and other xenobiotics.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2008年第12期1066-1069,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家重大基础研究前期专项(No.2002ccc00300)
关键词
肝损伤敏感基因
酒精
CCL4
卡介苗/脂多糖
黄药子
千里光
实验研究
Sensitive genes of liver injury
Alcohol
CCl4
BCG and LPS： Rhizoma Dioscoreae Bulbiferae
Senecio scandens Buch.-Ham
Experimental study
