摘要
以转B t基因棉花"国抗22"和常规棉亲本"泗棉3号"为试材,研究外源B t基因导入对棉花叶片维管束汁液中营养物质和次生物质含量以及烟粉虱种群增殖的影响。结果表明,和"泗棉3号"相比,"国抗22"叶片维管束汁液中可溶性糖含量较低;单宁浓度苗期较低,但花铃期两个品种无明显差异;花铃期游离氨基酸总量无明显差异,但谷氨酸、脯氨酸、丙氨酸含量均明显高于"泗棉3号";在苗期和花铃期两品种棉花叶片维管束汁液中均未检测到棉酚。取食苗期和花铃期"国抗22"棉花的B型烟粉虱内禀增长率rm分别比取食"泗棉3号"对应生育期的高13.7%和20.2%。研究表明,外源B t基因的导入影响转基因棉花中可溶性糖、游离氨基酸和其他抗生物质的合成,从而影响烟粉虱的种群发展。
Using transgenic Bt cotton ＂Guokang 22＂ and conventional parental cotton ＂Simian No.3＂ as tested varieties,the effect of introducing Bt gene on physiological indices of vascular bundle sap of cotton leaf and reproduction of Bemisia tabaci（Gennadius） was studied.The results show that transgenic Bt cotton ＂Guokang 22＂ contains more soluble sugar in vascular bundle sap both at seedling and flowering stages than the conventional parental cotton ＂Simian No.3＂.Tannin content in vascular bundle sap of ＂Guokang 22＂ is lower than that of ＂Simian No.3＂ at seedling stage,but not significantly different at flowering stage.Although no significant difference exists in total free amino acid content between two varieties,some amino acids（including glutamic acid,proline and alanine） that are closely related to B.tabaci metabolism are significantly higher in ＂Guokang 22＂ than in ＂Simian No.3＂ at flowering stage.No gossypol is detected from the sap at both seeding and flowering stages.The intrinsic rates of increase（rm） of B-type B.tabaci feeding on the leaves of trangenic Bt cotton ＂Guokang 22＂ at seeding and flowering stages are respectively 13.7% and 20.2% higher than those feeding on conventional parental cotton ＂Simian No.3＂.This indicates that the introduction of foreign Bt gene produces profound effect on the syntheses of soluble sugars,free amino acids and anti-biosies,and thus on the population of B.tabaci.
出处
《中国生态农业学报》
CAS
CSCD
2008年第6期1508-1512,共5页
Chinese Journal of Eco-Agriculture
基金
国家自然科学基金项目(30270901)
国家"十五"科技攻关项目(2001BA509B0604)
国家科技部转基因植物重大专项课题(2008ZX08012-004)
江苏省自然科学基金(BK2006067
BK2008056)
江苏省教育厅自然科学基金(07KJB210134)资助
关键词
Bt基因棉花
烟粉虱
叶片维管束汁液
营养物质
抗生物质
内禀增长率
Transgenic Bt contton,Bemisia tabaci（Gennadius）,Vascular bundle sap,Nutrients,Anti-biosies,Intrinsic rate of increase
