摘要
目的:测定孕妇胎盘组织和血清合体滋养细胞层微绒毛膜(STBM)在重度子痫前期的表达情况,揭示早发型与晚发型重度子痫前期可能具有不同的病因及发病机制。方法:收集在我院治疗的早发型与晚发型重度子痫前期患者各15例,及与两组孕周相匹配正常妊娠孕妇各10例。采用实时荧光定量PCR(Real-time RT-PCR)方法测重度子痫前期患者胎盘中STBM的标记物TPAmRNA水平,另用酶联免疫分析法(ELISA)测定血清和胎盘中STBM的标记物TPA蛋白表达水平。结果:①早发型组孕妇胎盘STBM的标记物TPA相对含量(2.04±0.32 ng/ml),高于晚发型组(1.75±0.31 ng/ml)(P<0.05),早发型组胎盘STBM的标记物TPA mRNA含量(0.0342±0.0021)高于晚发组(0.0222±0.0020)(P<0.05);②早发型组孕妇血清中STBM的标记物TPA水平(1.88±0.43 ng/ml)高于晚发型组(1.59±0.26 ng/ml)(P<0.05)。早发型组和晚发型组血清中STBM的标记物TPA水平均高于同期正常孕妇血清中的TPA水平(P均<0.05)。③早发型组孕妇胎盘组织中STBM的标记物TPA水平与血清尿素氮水平、血清肌酐水平、S/D比值之间均呈显著正相关。晚发型组中与血清肌酐水平之间呈显著正相关。④早发型组孕妇血清中STBM的标记物TPA水平与血清肌酐水平、收缩压、舒张压之间均呈显著正相关。晚发型组中与血清肌酐水平、S/D比值、收缩压之间均呈显著正相关。结论:早发型重度子痫前期胎盘的合体滋养细胞凋亡早且严重,早发型与晚发型重度子痫前期可能具有不同的病因及发病机制。
Objective：To explore different pathogeny and pathogenesis between early-onset and late-onset severe preeclampsia by analyzing the levels of syncytiotrophoblast microvillous membrane （STBM） in the placenta and serum of such patients. Methods： Both early-onset and late-onset severe preeclampsia group had 15 patients; each had 10 normal pregnancies with matching gestational age as control. TPA mRNA level were tested by Real-time RT-PCR. Senun and placenta TPA were tested by ELISA. Results：（1）Placenta TPA concentration in early-onset group were significantly higher than those in late-onset group（2.04 ± 0.33 ng/ml VS 1.75 ±0.31 ng/ml, P 〈 0.05 ）. Placenta TPA mRNA concentration in early-onset group were significantly higher than those in late-onset group （0.0342 ± 0.（X）21 VS 0.0222 ± 0.0020, P〈 0.05）. QSerum rPA level in early-onset group were significantly higher than those in late-onset group （ 1.88 ±0.43 ng/ml VS 1.59 ± 0.26 ng/ml, P 〈 0.05）. Serum TPA level in early-onset and late-onset groups wore significantly higher than those in control groups（ P 〈 0.05）. Q Placenta TPA level correlated positively with serum urea nitrogen,creatinine level and S/D in early-onset group,but correlated positively only with serum creatinine level in the late-onset group.（1）Serum TPA level correlated positively with the serum creatinine level,the systolic blood pressure,the diastolic blood pressure in early-onset group and correlated positively with the serum creatinine level,S/D, the systolic blood pressure in the late-onset severe preaclampsia. Conclusions： The placental syncytiotrophoblast have earlier and more serious apoptosis in early-onset severepreeclarnpsla, indicating that early-onset and late-onset severe preeclampsia may have different pathogeny and pathogenesis.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2008年第11期677-680,共4页
Journal of Practical Obstetrics and Gynecology
关键词
早发型重度子痫前期
合体滋养细胞层微绒毛膜
胎盘
Early-onset severe preeclampsia
Syncytiotrophoblast microvillous membrane
Placenta
