摘要
背景:失用性骨质疏松由于肌肉不活动和负重减少引起骨量丢失。目的:观察失用性骨质疏松发展过程中不同时间内骨的结构、矿物质含量以及受其影响骨生物力学性能的变化。设计、时间及地点:对照观察动物实验于2003-09/11在天津医科大学总医院完成。材料:雄性8月龄日本大耳白兔 55 只。方法:选用雄性日本大耳白兔55只。随机取50只兔,石膏管型固定右后肢作为实验侧,左侧后肢不固定作为对照侧,另5只双侧后肢作为空白对照组饲养 3个月。主要观察指标:取双侧胫腓骨行力学试验,测定钙元素的含量。取双侧跖骨头以苏木精-伊红染色和Masson染色进行组织学观察。结果:对于最大负荷、弹性模量和骨钙含量等指标,实验侧胫腓骨在不同固定时间的差别显著,实验侧和空白对照组间均有差别(P<0.05);对照侧和空白对照组间的差别不显著(P>0.05)。实验侧骨皮质变薄,孔隙增多,骨小梁稀疏、纤细并有多处断裂,髓腔扩大,破骨细胞数增多。Masson染色见随着失用时间延长,新生胶原量也在减少。对照侧和空白对照组跖骨头骨皮质较厚,孔隙较少,骨小梁排列紧密,结构正常。结论:在失用性骨质疏松形成过程中,骨钙含量、骨整体生物力学性能持续下降,但速度趋缓,以适应所处应力环境和功能状态。
BACKGROUND： Disuse osteoporosis is bone mass loss caused by muscular inactivity and reduced weight bearing. OBJECTIVE： To observe bone structure and mineral matter content as well as bone biomechanical performance change at different time points during the development of disuse osteoporosis. DESIGN, TIME AND SETTING： The present randomized, controlled, and self-controlled animal experimental was performed in the General Hospital of Tianjin Medical University between September and November 2003. MATERIALS： A total of 55 male Japanese rabbits, aged 8 months, weighing 2.5 3.0 kg were enrolled for this study. METHODS： The right hind limbs of 50 randomly selected rabbits were immobilized by cast （experimental sides）. The corresponding left hind limbs were untouched （control sides）. The remaining 5 rabbits were raised for 3 months, and their bilateral hind limbs were used for controls. Ten rabbits were sacrificed by intravenous injection of air at each time point （2, 4, 6, 8, and 12 weeks after immobilization）. MAIN OUTCOME MEASURES： Mechanical test was performed on bilateral tibiofibula for determination of calcium content. Hematoxylin-eosin staining and Masson staining of bilateral metatarsal bones were performed for histological observation. RESULTS： There was significant difference in indices of tibiofibula on the experimental sides, including maximum loading, elastic modulus, and calcium content among different time points, while signifcant difference also existed between the experimental side and the blank controls （P 〈 0.05）. There was no significant difference in above-mentioned indices between the control side and blank controls as well as between control sides （P 〉 0.05）. The experimental side limbs exhibited thinned cortical bone, increased pores, sparse and fine trabecular bone with several fragmentations, expanded medullary cavity, and increased osteoblasts. Masson staining results demonstrated that with disuse time going, collagen was decreasingly formed. The control
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第46期9006-9009,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
the Natural Science Foundation of Tianjin City, No. 993607711~~
