摘要
目的探讨碘对小鼠产后甲状腺炎（postpartum thyroiditis，PPT）发生、发展的影响。方法44只8周龄雌性C57BL／6J小鼠均饲以低碘饲料（含碘量≤35μg/kg），按体质量随机分成4组：非妊娠实验性自身免疫性甲状腺炎（EAT）组8只，采用猪甲状腺球蛋白加完全弗氏佐剂复制EAT模型，最终存活6只；NI—PPT组（正常碘）、10HI—PPT组（10倍碘）、50HI—PPT组（50倍碘）各12只，复制EAT模型（方法同非妊娠EAT组）后，与性成熟雄鼠交配，分别有7、6、6只小鼠受孕。4组小鼠分别饮用含KI为0．3、0.3、3．0、15．0mg／L的碘水。妊娠母鼠生产4周后，观察4组小鼠甲状腺组织病理改变、血清中甲状腺球蛋白抗体（Tg-Ab）和甲状腺过氧化物酶抗体（TPO-Ab）水平、血清中甲状腺激素（TT3、TT4）水平以及脾脏中IFN-γ和IL-4mRNA的表达水平。结果甲状腺组织病理检查示甲状腺内炎细胞浸润，上皮细胞扁平，滤泡萎缩或破坏。非妊娠EAT组、NI-PPT组、10HI—PPT组小鼠的炎细胞浸润程度均低于50HI—PPT组，组间两两比较，差异有统计学意义（P〈0．05）。非妊娠EAT组、NI-PPT组、10HI-PPT组和50HI—PPT组的血清TPO-Ab水平分别为（14．32±8．85）％、（64．45±10．52）％、（38．46±5．57）％、（90．09±9．98）％，任意两组组间比较，差异均有统计学意义（P〈0．05）；血清Tg-Ab水平分别为（33．74±3．71）％、（29．65±2．06）％、（37．21±3．87）％、（33．87±4．17）％，组间比较差异无统计学意义（F=0．484，P〉0．05）；血清TT3水平分别为（2．47±0．69）％、（1．57±0．25）％、（1．60±0．28）％、（1．82±0．75）％，组间比较差异无统计学意义（F：1．596，P〉0．05）；非妊娠EAT组、NI—PPT组和10HI-PPT组血清中TT4水平[（99．87±5．97）％、（89．13±7．64）％、（91．05±5．82）％]与50HI—PPT组[（66．68±5．47）�
Objective To explore the effect of iodine on the pathogenesis of postpartum thyroiditis. Methods Forty-four female C57BL/6J mice, 8-week old, fed by low iodine dietary（the concentration of iodine≤35μg/kg）, were randomly divided into 4 groups： non-pregnancy experimental autoimmune thyroiditis （nonpregnancy EAT） group with 8 mice, EAT of mice was induced by immunization with pig＇s thyroglobulin（Tg） in the presence of complete Freund＇s adjuvant. Six mice in non- pregnancy EAT group survived at the end of experiment; normal iodine-PPT（NI-PPT） group, 10-fold high iodine-PPT（10HI-PPT） group and 50-fold high iodine-PPT（50HI- PPT） group with 12 mice in each group. The last 3 groups mice, who received the same immunization schedule as the above, were mated with adult male mice followed by induction of EAT. In the end,7,6 and 6 mice were noticed to be pregnant in each group. All animals were killed 4 weeks after postpartum. Histological severity of thyroid specimens was evaluated. The serum levels of thyroglobulin antibody（Tg-Ab）, thyroid peroxidase antibody （TPO-Ab）, TT3 and TF4 were measured by radioimmunoassay （RIA）. The expression level of IFN-γ/IL-4 mRNA in spleen were assayed by RT-PCR. Results Pathological examination showed the infiltration of inflammatory cells, epithelial cell applanation, follicle atrophy or destruction. The severity of inflammation in non-pregnancy EAT group, NI-PPT group and 10HI-PPT group was less serious than that in the 50HI-PPT group, the difference has statistical significance（P 〈 0.05）. The level of TPO-Ab in non-pregnancy EAT group, NI-PPT group, 10HI-PPT group and 50HI-PPT group was （14.32 ±8.85）%, （64.45 ±10.52）%, （38.46±5.57）% and （90.09± 9.98）%, respectively the difference being statistically significant between any two groups （P 〈 0.05）. There was no statistical difference （F = 0.484,P 〉 0.05） of Tg-Ab among non-pregnancy EAT group [ （33.74 ± 3.71）% ], NI-PPT group [（29.65±2.06）%], 10HI-PP
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2008年第6期617-621,共5页
Chinese Jouranl of Endemiology
基金
天津市科技发展计划项目（05YFGDSF02700）
天津市高等学校科技发展基金项目（2004ZD08）
关键词
碘
产后甲状腺炎
自身免疫
Iodine
Postpartum thyroiditis
Autoimmunity
