摘要
目的建立高效液相色谱检测茴三硫原料药含量的方法。方法采用Alltech Alltima C18色谱柱（4．6mm×250mm，5μm），以甲醇-水（85：15）为流动相，流速1．0mL·min^-1，检测波长348nm，进样量20μL。结果在该色谱条件下，茴三硫在4．064-101．60μg·mL^-1。浓度范围内呈良好的线性关系，A=61940C-18960．31（r=0．99992，n=7），平均回收率为99．6％（RSD=0．22％）。结论本法灵敏、准确、回收率高、操作简便，可作为茴三硫含量的检测方法。
OBJECTIVE To establish a HPLC method for determination of the contents of Anethole trithione.METHODS The separation and analysis was performed on a C18 column（4.6mm × 250mm, 5μm）, the mobile phase consisted of methanol-water （85： 15）, the flow rate was 1.0mL· min^-1, the detection wavelength was set at 348nm, loaded volume was 20μL. RESULTS A good linear relationship was obtainted in the range of 4. 064 - 101.60μg· mL^- 1, A = 61940C-18960.31 （ r = 0. 99992, n = 7）. The average recovery rate was 99.6 % （RSD = 0.22 % ）. CONCLUSION The proposed method is sensitive, accurate and simple, and can be used for the determination of the contents of Anethole trithione.
出处
《海峡药学》
2008年第11期34-36,共3页
Strait Pharmaceutical Journal
关键词
茴三硫
原料药
含量
高效液相色谱法
Anethole trithione
Bulk drug
Contents
High performance liquid chromatography（ HPLC ）
