摘要
为了研究Ca2+对高温强光胁迫下小麦光合机构的保护机理,分别用清水和10 mmol/L的CaCl2水溶液预处理灌浆期的小麦叶片,然后进行不同的温度和照光处理,探讨了Ca2+对高温强光胁迫下小麦叶片D1蛋白和PSⅡ功能的影响。结果表明,叶面喷施Ca2+有效地调节了高温强光下抗氧化酶SOD、APX的活性,降低了叶片MDA的含量和相对电导率;Ca2+处理维持了较高的最大光化学效率(Fv/Fm)和较低的原初荧光(Fo),减轻了逆境对光合机构的破坏。Western Blotting分析结果也显示,Ca2+减轻了高温强光对小麦叶片PSⅡ反应中心D1蛋白的破坏。
The protective mechanism of Ca^2＋ on photosynthetic apparatus of wheat leaves under heat and high irradiance stress were investigated. The leaves of wheat were sprayed by solution of CaCl2 （10 mmol/L） or water （as control） at grain-filling stage and exposed to different temperature and irradiance. The effect of Ca^2＋ on D1 proteins and photosystem Ⅱ（PSⅡ） performance of wheat leaves under heat and high irradiance stress were studied. The results showed that pretreatment with Ca^2＋ enabled the wheat leaves to keep relatively high activities of superoxide dismutase （SOD） and ascorbate peroxidase （APX）, lower malondialdehyde （ MDA ） content and relative electrical conductivity,higher Fv/Fm and lower Fo. The analysis of Westen Blotting indicated that Ca^2＋ pretreatment can decrease the degradation of D1 proteins.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2008年第6期1042-1047,共6页
Journal of Triticeae Crops
基金
国家自然科学基金项目(30671214)
关键词
小麦
钙离子
高温强光胁迫
抗氧化酶
D1蛋白
Wheat
Calcium ion
Heat and high irradiance stress
Antioxidant enzyme
D1 protein
