摘要
中国大鲵是世界上最大的两栖动物并且为我国特有,现在该物种野生种群急剧下降,而人工养殖种群逐渐增多。为了对大鲵(Andrias davidianus)群体进行遗传多样性的本底调查,本文用10对微卫星引物对28尾野生大鲵和16尾人工养殖的大鲵样本进行了遗传多样性分析。结果表明,在10对引物中有7对检测到多态位点,野生群体和养殖群体的观察等位基因数分别为5-8和4-6,期望杂合度分别为0.81和0.75,说明本实验中研究的大鲵的遗传多样性水平较高。通过人工养殖群体和野生群体的比较发现,人工养殖群体存在较大的等位基因丢失现象,并且遗传多样性水平低于野生群体。以上结果将为大鲵的人工繁育和遗传多样性的保护、利用提供一定的理论依据。
The Chinese giant salamander （Andrias davidianus） is world＇s largest amphibian and is endemic to China. Wild populations of the species have declined drastically, while domesticated populations have increased in recent years. To estimate genetic variation in the Chinese giant salamander, we analyzed ten microsatellite loci of 44 individuals from two wild and one domesticated populations. A total of 52 alleles were found from seven loci shown to be polymorphic. The number of alleles ranged from 6 to 9 （mean 7.4）. The polymorphism information content （PIC） of all populations at all loci exceeded 0.54 except the YQY at GS134 （0.3750）, suggesting high polymorphism at microsatellite markers. Compared with the wild populations, some alleles in the domesticated population were drifted and the PIC was lower. Our results may provide a theoretical basis for conservation and exploitation of giant salamanders in China.
出处
《生物多样性》
CAS
CSCD
北大核心
2008年第6期533-538,共6页
Biodiversity Science
基金
农业科技成果转化项目(05EFN216900350)
国家科技支撑计划专项(2006BAD03B08-04)
国家科技基础条件平台专项(2006DKA30470-002)
