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复方红景天对大鼠肝纤维化肝脏中TIMP-1表达的影响 被引量:11

Effect of Chinese herbs compound Rhodiola sachalinensis on the expression of tissue inhibitor of metalloproteinase1 in fibrotic fiver in rats
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摘要 目的:探讨复方红景天对四氯化碳(CCl4)诱导的大鼠肝纤维化肝组织TIMP-1基因表达的影响.方法:健康♂SD大鼠80只随机分组:(1)正常对照组;(2)肝纤维化模型组;(3)复方红景天高剂量组(H组);(4)复方红景天中剂量组(M组);(5)复方红景天低剂量组(S组),每组16只.以CCl4ip法诱导大鼠肝纤维化,复方红景天干预组大鼠在造模的同时给予复方红景天灌胃,正常对照组给予橄榄油sc和生理盐水ip,8wk实验结束时处死动物,留取的肝脏组织做HE按0-4期标准判定肝纤维化程度,应用半定量RT-PCR检测肝组织中TIMP-1 mRNA的表达情况,用免疫组化检测肝组织中TIMP-1的表达.结果:模型组大鼠肝纤维化程度处于2-4期,其中大部分达3期以上,复方红景天干预组大鼠肝纤维化程度明显减轻,只有少部分达3期.模型组大鼠肝组织中TIMP-1 mRNA水平高于正常对照组(0.858±0.052 vs 0.615±0.067,P<0.05),而复方红景天干预组大鼠肝组织中TIMP-1 mRNA水平(0.740±0.081,0.704±0.032,0.695±0.030)显著低于模型组(均P<0.05);模型组大鼠肝组织TIMP-1的阳性表达显著强于正常对照组(0.356±0.052 vs 0.121±0.067,P<0.05),复方红景天干预组肝组织中TIMP-1的阳性表达(0.298±0.081,0.256±0.032,0.213±0.030)明显强于正常对照组(均P<0.05),但较模型组显著减弱(P<0.05).结论:中药复方红景天能有效抑制CCl4诱导的肝纤维化大鼠肝脏中TIMP-1的表达. AIM: To observe the change of tissue inhibitor of metaIloproteinase-1 (TIMP-1) expression in carbon tetrachloride-hnduced liver fibrosis in rats after treatment with Chinese herbs compound Rhodiola sachalinensis (CHCRS). METHODS: Eighty healthy male SD rats were randomly divided into 5 groups (n = 16, for each group): normal group, fibrosis group, CHCRS- treated groups (using high-, middle- and low- dose CHCRS, respectively ). Liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (for 8 wk). CHCRS was given intragas trically twice a week while fibrosis was induced. Animals were sacrificed at the end of experiment. Histological examinations were performed after hematoxylin and eosin staining, and the fibrosis was classified into stage 0 to 4. The level of he- patic TIMP-1 mRNA was determined by semiquantitive RT-PCR, and the expression of TIMP-1 protein was detected by immunohisfochemistry. RESULTS: The extent of liver fibrosis was above the 3th stage in most model animals, but in CHCRS-treated rats, it was obviously degraded as compared with that in the model group. The level of hepatic TIMP-1 mRNA was significantly higher in the model group than that in the normal group (0.858 ± 0.052 vs 0.615 ± 0.067, P 〈 0.05), but it was markedly lower in the CHCRS- treated groups than that in the model group (0.740 ± 0.081, 0.704 _± 0.032, 0.695 ± 0.030; all P 〈 0.05). Immunohisochemistry showed a signifi- cant increase of TIMP-1 in the model group as compared with that in the normal group (0.356 ± 0.052 vs 0.121 ± 0.067, P 〈 0.05), but a notable decrease in the CHCRS-treated groups as com- pared with that in the model group (all P 〈 0.05). Meanwhile, the positive expression of TIMP-1 in liver tissues of CHCRS-treated groups was still stronger than that in the normal group (0.298 ± 0.08, 0.256 ± 0.032, 0.213 ± 0.030; all P 〈 0.05). CONCLUSION: CHCRS can efectively inhibit the expression of TIMP-1 in CC14-induced fibrotic liver
出处 《世界华人消化杂志》 CAS 北大核心 2008年第31期3543-3546,共4页 World Chinese Journal of Digestology
基金 黑龙江省卫生厅科研资助项目 No.2006-337 黑龙江省科技攻关资助项目 No.PC06S15~~
关键词 复方红景天 肝纤维化 TIMP-1 免疫组化 逆转录-聚合酶链反应 Chinese herbs compound Rhodiolasachalinensis Liver fibrosis Tissue inhibitor of met-alloproteinase-1 Immunohistochemistry Reversetranscription-polymerase chain reaction
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