摘要
目的了解急性下呼吸道感染(ALRI)住院患儿病毒感染情况及其临床特征。方法收集2004-2006年冬季(11月至次年2月)因ALRI在北京儿童医院住院治疗的<5岁患儿鼻咽吸出物333份。男230例,女103例;年龄1个月～4.8岁。采用PCR技术和病毒分离方法对鼻咽吸出物进行呼吸道合胞病毒(RSV)、腺病毒(AdV)、副流感病毒(PIV)1～4型、鼻病毒(RhV)、人偏肺病毒(hMPV)、冠状病毒(HCoV)及人博卡病毒(HBoV)检测。采用人喉上皮癌细胞和非洲猴肾细胞对鼻咽吸出物进行病毒分离,对阳性结果进行序列测定和鉴定。结果ALRI333例患儿中,病毒检测阳性123份(36.94%),其中RSV阳性102例,AdV、HBoV阳性各16例,RhV阳性1例,其中12例为双重阳性。未检测到PIV、hMPV及HCoV。333份鼻咽吸出物中病毒分离得到11株病毒株,经PCR扩增及序列测定证实均为AdV,包括AdV1型2株,AdV2型、AdV3型、AdV7型各3株。肺炎患儿病毒阳性检出率为30.35%,毛细支气管炎患儿病毒阳性检出率为62.50%,急性支气管炎患儿病毒阳性检出率为23.08%,毛细支气管炎患儿病毒阳性检出率高于肺炎和急性支气管炎患儿(χ2=24.75,19.66Pa<0.01)。结论RSV感染在北京儿童医院2004-2006年冬季ALRI住院患儿中占首位;感染的AdV血清型包括AdV1型、AdV2型、AdV3型及AdV7型;HBoV可能是ALRI的重要病原。毛细支气管炎患儿病毒感染检出率最高。
Objective To investigate the respiratory viruses and their clinical characteristics in hospitalized children with acute lower respiratory tract infection （ALRI）. Methods A total of 333 nasopharyngeal aspirates were collected from children suffering from ALRI during winters from 2004 to 2006 （ Nov. through the following Feb. ）. They were 230 boys and 103 girls with age ranging from 1 month to 4.8 years. The respiratory viruses, including respiratory syncytial virus （ RSV）, adenovirus （ AdV）, parainflueuza （ PIV ） 1 - 4, rhinovirus （ RhV）, human metapneumovirus （hMPV）, human eoronavirus （HCoV） and human bocavirus （HBoV） were detected by using polymerase chain reaction amplification and viral isolation. Viral isolation was performed using HEp - 2 cell and Vero cell. Positive amplification products and isolates were sequenced for identification. Results Overall at least one respiratory virus was detected in 123 of 333 children （36.94%） by at least one technique,102 samples were RSV positive,16 samples were AdV positive,16 samples were HBoV positive and 1 sample was RhV positive, double viral infections were present in 12 of 333 samples. PIV, hMPV and HCoV were not detected in all samples by PCR and viral isolation. Eleven isolates performed using viral isolation were obtained from 333 samples. These 11 isolates were all AdV confirmed by PCR and se- quence identification,including AdV1 2 strains, AdV2 3 strains ,AdV3 3 strains and AdV7 3 strains. Among patients with pneumonia,30.35% were shown to be viral positive. Among patiems with bronchiolitis,62.50% were shown to be viral positive. Among patients with bronchitis, 23.08% were shown to be viral positive. The detection rate in children with bronchiolitis was significantly higher than that in children with pneumonia or bronchitis （x^2 = 24.75,19.66 Pa 〈 0.01 ）. Conclusions RSV was the first common viral pathogen in children hospitalized with ALRI in Beijing children＇s hospital during winters from 2004 to 2006. There wer
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2008年第22期1743-1744,1756,共3页
Journal of Applied Clinical Pediatrics
基金
国家高技术研究发展计划项目资助(2007AA02Z463-3)
关键词
病毒
博卡病毒
呼吸道感染
聚合酶链反应
儿童
virus
bocavirus
respiratory tract infection
polymerase chain reaction
child
