摘要
目的建立伯氏疏螺旋体感染的小鼠模型,研究伯氏疏螺旋体在小鼠关节组织中的关节特异性基因表达谱。方法首先,建立伯氏疏螺旋体感染小鼠模型,在不同时间点处死小鼠,收集小鼠关节、心脏、皮肤和膀胱组织,从四种组织中分别提取总RNA;随后,用DECAL技术和微阵列技术分析不同时间点伯氏疏螺旋体在四种组织中的转录组;最后,将伯氏疏螺旋体在关节中的转录组依次与其他三种组织中的转录组进行比较,找出仅在关节中表达的伯氏疏螺旋体基因,从而获得伯氏疏螺旋体不同时间点的关节特异性基因表达谱。结果与其他组织相比,在感染后第15天,伯氏疏螺旋体在小鼠关节组织特异地表达21个基因,其中13个基因位于伯氏疏螺旋体染色体上,8个基因位于质粒上;在感染后的第105天,伯氏疏螺旋体在小鼠关节组织特异地表达24个基因,其中13个基因位于伯氏疏螺旋体染色体上,11个基因位于质粒上。结论伯氏疏螺旋体在小鼠关节组织中存在独特的基因表达谱,这些在关节中特异表达的基因可能与莱姆关节炎的发生、发展有关。
Objective To identify the joint-specific expression profile of Borrelia burgdorfri in the murine hosts. Methods Establishing the murine model of Lyme disease, sacrificing the infected mice at different time-points, collecting the joint, heart, skin, and urinary bladder samples of the infected mice, and extracting total RNA from the samples. DECAL technique and microarray were applied to btain the transcriptomes of Borrelia burgdorferi in the joints, heart, skin, and urinary bladder, the transcriptomes from different tissues were compared to identify the jointspecific expression profile of Borrelia burgdorferi. Results Borrelia burgdorferi expresses 21 joint-specific genes, including 13 genes located in chromosome and 9 genes located in plasmids at day 15 after infection, and expresses 24 genes, including 13 genes in chromosome and 11 genes in plasmids at day 105 after infection. Borrelia burgdorferi shows specific gene expression profile in the murine joints. Conclusion Borrelia burgdorferi expresses some specific genes different from those in the other tissues in infected murine joints. These joint-specific genes might were involved in the survival and arthritis pathogenesis of Borrelia burgdorferi.
出处
《科技通报》
2008年第6期832-838,846,共8页
Bulletin of Science and Technology
基金
Supported by the USA NIH(No.:5R21AI054630-02)
