摘要
目的:用PCR方法检测尿道致病性大肠埃希菌(UPEC)的papC基因,探索建立UPEC的鉴定方法和筛选特异分子标识。方法:根据已发表的papC基因序列设计引物,采用PCR方法扩增460 bp左右的papC基因,并与血凝试验相比较,用于尿道致病性大肠埃希菌的检测。结果:156株尿源性大肠埃希菌中MRHA阳性菌株的检出率为31.41%,pap CPCR扩增的阳性率为32.05%,两组结果无显著性差异(P>0.05)。结论:papC PCR方法可用于UPEC菌株的快速、灵敏的检测,其扩增片段可作为分子标识用于UPEC的筛选。
Objective：To detect papC gene with polymerase chain reaction in order to establish an assay to identify UPEC strains and screen of the molecular marker from Uropathogenic Escherichia coli strains, netheds： A pair of oligonucleotide primers derived from the published DNA sequence of papC gene were used in PCR to emplify 460bp - long papC gene. As compared with hemagglutination test, PCR methed was applied to detect papC gene of UPEC strains. Results ：The detection rates of MRHA and papC PCR positive strains of 156 E. coli stains isolated from urine of patients with urinary tract infections were 31.41% and 32. 05%, respectively. There was no statistical significance between the above results （P 〉 0. 05 ）. Conclusion：The papC assay is the specific and sensitive method in the detection of UPEC strains and the 460 bp sequence may be proved to be valuable for the identification of UPEC strains as the specific molecular marker.
出处
《中国卫生检验杂志》
CAS
2008年第11期2234-2235,2294,共3页
Chinese Journal of Health Laboratory Technology
