摘要
用PCR方法克隆了山羊痘病毒疫苗株(GTPV-TY)的TK基因,根据TK基因结构,设计了2对引物分别扩增得到与TK基因部分相邻的长度约1 kb的同源重组臂序列,分别插入到pGEM-T easy载体,通过SmaⅠ位点重新连接,并在此位点插入CMV启动子、多克隆位点、BGH polyA信号以及LacZ基因,构建了通用山羊痘病毒TK基因缺失转移载体pdTK。此转移载体为改造GTPV-TY株以及开发二价或多价基因工程疫苗奠定了基础。
The thymidine kinase gene of goat pox virus was cloned by PCR. The sequences near the TK genes used as homologous recombinant arms which were about lkb were amplified by PCR with primer pairs, and they were inserted into pGEM-T easy vector. The goat pox virus deleted TK transfer vector pdTK were constructed by re-linked the homologous recombinant arms on the Sma Ⅰ site, and CMV promoter, multiclone sit, BGH polyA, LacZ genes were inserted into the Sinai site. This recombinant transfer vector are expected to be useful for the development of GTPV-TY gene engineering vaccine.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2008年第5期739-742,共4页
Journal of Jilin Agricultural University
基金
国家“973”计划项目(2005CB523202)
