摘要
通过室内试验,研究了用LC50及LC90两种浓度敌百虫汰选14代建立的不同程度高抗性水平的桔小实蝇Bactrocera dorsalis抗性品系TrR50和TrR90在停止使用药剂后的抗性衰退规律,以及抗性衰退到原有水平的1/3时再用药汰选的抗性再增长规律。结果表明:两抗性品系对敌百虫抗性均不稳定,但抗性衰退速率不同,TrR90完全隔离药剂4代后,抗性已衰退至原有水平的1/3,而TrR50完全隔离药剂7代后,抗性才衰退至原有水平的1/3,但随后两品系抗性衰退均减缓,到19代后抗性均还处于低水平抗性阶段,无法衰退至敏感水平。通过对其抗性衰退趋势进行方程拟合,结果表明两品系抗性衰退均符合S型曲线模型。抗性再增长试验结果表明:桔小实蝇两抗性品系对敌百虫抗性再增长趋势不同,TrR50继续使用药剂汰选,经过6代选育,抗性迅速上升,接近原有的抗性水平,随后保持平稳增长;而TrR90继续使用药剂汰选,经过9代选育,抗性才迅速上升,经过12代选育,抗性才接近原有的抗性水平。通过对两品系抗性再增长趋势进行方程拟合,结果表明两品系抗性再增长趋势不同,TrR50品系抗性再增长趋势符合逻辑斯蒂模型,而TrR90品系则符合二次曲线模型。
In the laboratory, two different high-level resistant strains TrR50 and TrR90 of the oriental fruit fly, Bactrocera dorsalis to triehlorphon were established after 14-generation selection by using the concentrations of LC50 and LC90 of trichlorphon, the TrR50 and TrR90 strains without exposure to any insecticides were kept for 19 generations, and the resistance recession to trichlorphon were studied. At the same time, the re-growth resistance was monitored after the two strains were bred for 4 generations under insecticide-free condition, and then selected with trichlorphon for 12 generations. The results showed that the resistance of the two resistant strains was all instable, but their depression rates were different. After TrR90 and TrR50 were selected for 4 and 7 generations respectively, their resistance decreased to one third of the original level. Then the resistance of the two strains was depressed slowly, and after the two strains were selected for 19 generations, their resistance kept at a low level but was unable to be depressed to the susceptible level. Through equation fitting of resistance depression tendency, it was found that the resistance depression of the two strains was both in line with S-curve model. The resistance re-growth of the two strains was different. After selection with trichlorphon for 6 generations, TrR90 resistance went up rapidly which was close to the original level; but only after selection for 9 generations with trichlorphon, TrR90 resistance began to increase rapidly, which was close to the original level only after 12-generation selection. The results of the equation fitting of resistance re-growth tendency showed that resistance re-growth of the two strains was quite different, and the fitting curve of TrR50 strain was in line with the logistic model, while that of TrRgo was in line with quadratic curve model.
出处
《昆虫学报》
CAS
CSCD
北大核心
2008年第10期1044-1049,共6页
Acta Entomologica Sinica
基金
广东省科技计划项目(2002B2160203,2004A20401002)
农业部项目(农农植保字[2006]003号)
广东省桔小实蝇为害控制专项(粤财农[2003]97,[2004]92,[2005]358)
关键词
桔小实蝇
敌百虫
抗药性
抗性稳定性
抗性再增长
Bactrocera dorsalis
trichlorphon
insecticide resistance
resistance stability
resistance re-growth
