摘要
目的:研究培哚普利对氧化修饰的低密度脂蛋白(ox-LDL)诱导的U937泡沫细胞血管内皮生长因子(VEGF)mRNA、蛋白表达的影响。方法:U937细胞与80μg/mLox-LDL孵育48h,建立U937泡沫细胞模型,以不同浓度的培哚普利(0.01、0.10、1.00μmol/L)预处理U937细胞24h再加入80μg/mL的ox-LDL孵育48h,通过逆转录-聚合酶链式扩增反应(RT-PCR)和酶联免疫吸附试验(ELISA)分别检测U937细胞VEGF mRNA和蛋白的表达情况。结果:U937泡沫细胞组VEGF mRNA的表达较U937细胞对照组明显增加[(2.371±0.253)vs(0.954±0.245)(P<0.01)],培哚普利干预后,随着浓度(0.01、0.10、1.00μmol/L)的增加,U937泡沫细胞的VEGF mRNA表达水平呈浓度依赖性下降[(2.168±0.270)vs(1.533±0.248)vs(1.022±0.189)(P<0.01)];U937泡沫细胞组VEGF蛋白表达较U937细胞对照组明显增加[(1804.18±177.59)pg/mL vs(716.19±60.82)pg/mL(P<0.01)],培哚普利干预后,随着浓度(0.01、0.10、1.00μmol/L)的增加,U937泡沫细胞VEGF蛋白表达呈浓度依赖性降低(1601.46±154.68)pg/mL vs(1377.09±110.36)pg/mL vs(1017.89±147.18)pg/mL(P<0.01)。结论:培哚普利能够浓度依赖性地下调ox-LDL诱导的U937泡沫细胞VEGF mRNA、蛋白的表达。
Objective： To study the expression of vascular endothelial growth factor （VEGF） induced by oxidized low density liprotein （ox-LDL） and the inhibitory effects of perindopril on the levels of VEGF protein and mRNA in the U937 foam cells. Methods： The U937 cells were incubated with ox-LDL 80 μg/mL for 48 h and a macrophage-derived foam cell model was established. The medium was pretreated with perindopril （0.01,0.1,1 μmol/L） for 24 h , incubated with ox-LDL 80 μg/mL for 48 h. Reverse transcription-polymerase chain reaction （RT-PCR） and enzyme-linked immuneosorbent assay （ELISA） were applied to determine the expression of VEGF- protein and mRNA in U937 ceils and treated U937 cells. Results： VEGFmRNA levels in U937 cells after incubation with ox-LDL 80 μg/mL increased signiflcandy compared with control cells（foam cells vs control cells, 2.371±0. 253 vs 0. 954±0. 245, P 〈 0.01 ）. After treated with perindopril （0.01,0.10,1.00 μmol/L）, VEGF protein levels were coneentration-depently attenuated（foam ceils vs perindopril treated ceils, 2.371 ± 0.253 vs 2. 168 ± 0. 270,1. 533 ±0.248,1. 022 ±0. 189, P 〈0.01 ）. VEGF protein levels in the medium after incubation with ox-LDL 80 μg/mL increased significantly compared with control ceils（ foam cells vs control cells , 1 804.18 ± 177.59 pg/ mL vs 716.19 ± 60.82 pg/mL, P 〈 0.01 ）. After treated with perindopril（0.01, 0.10, 1.00 μmol/L）, VEGF protein levels were concentration-depently attenuated（foam cells vs perindopril treated cells, 1 804.18 ± 177.59 vs 1 601.46 ± 154.68 pg/mL, 1 377.09 ± 110.36 pg/mL, 1 017.89 ± 147.18 pg/mL, P 〈 0.01 ）. Conclusion： Perindopril can signifantly attenuated VEGF mRNA and protein expression in foam cells.
出处
《海南医学院学报》
CAS
2009年第1期10-14,共5页
Journal of Hainan Medical University
关键词
培哚普利
泡沫细胞
血管内皮生长因子
Perindopril
Foam ceils
Vascular endothelial growth factor
