摘要
目的研究虎杖提取物防治急性痛风性关节炎的作用机理。方法采用尿酸钠结晶(MSU)致急性痛风性关节炎大鼠模型,应用免疫组化法测定黏附分子(ICAM-1)和核转录因子(NF-κBp65)蛋白的阳性表达面积和积分光密度值,观察虎杖提取物对滑膜组织中ICAM-1和NF-κBp65表达的影响。结果正常组有少量表达,模型组I-CAM-1和NF-κBp65蛋白表达显著增加,虎杖提取物360mg/kg组可显著减少ICAM-1和NF-κBp65蛋白的阳性表达面积及积分光密度值。结论虎杖提取物可能是通过抑制大鼠滑膜组织中ICAM-1和NF-κBp65的异常表达与激活来发挥对痛风性关节炎的防治作用。
Objective To study the therapeutic mechanism of Polygonum cuspidatum extract on the gouty arthritis induced by monosodium urate (MSU). Method Acute gouty arthritis rats model was induced by MSU. Twelve hours after modeling,the positive expression area and integral optical density of intercellular adhesion molecule-1 (ICAM-1) and nuclear factor-κB p65 protein (NF-κBp65) in synovial membrane were observed by immunohistochemical assay. The effect of Polygonum cuspidatum extract on ICAM-1 and NF-κBp65 was observed. Results There existed less expression of ICAM-1 and NF-κB p65 protein on synovial membrane in the normal group,and their expression was much increased in the model group. Polygonum cuspidatum extract in the dose of 360 mg/kg significantly reduced the positive expression area and integral optical density of ICAM-1 and NF-κBp65 protein. Conclusion Polygonum cuspidatum extract can inhibit the abnormal expression and activation of ICAM-1 and NF-κBp65 in synovial tissue,which may be an important therapeutic and preventive mechanism for gouty arthritis.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第6期434-437,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
虎杖提取物
急性痛风性关节炎
黏附分子
核转录因子
Polygonum cuspidatum extract
Acute gouty arthritis
Adhesion molecule
Nuclear factor
