摘要
报道了青霉素酰化酶法制备D-丙氨酸的研究结果,为D-丙氨酸的制备提供了一种方法。考察了pH、温度、DL-丙氨酸与苯乙酰氯的摩尔比、反应时间对制备结果的影响。确定了制备N-苯乙酰-DL-丙氨酸的最佳反应条件:pH=9.5,n(DL-丙氨酸)∶n(苯乙酰氯)=1.1∶1;酶促反应最佳条件为:pH=7.5,温度37℃条件下反应6h,其产物为N-苯乙酰-D-丙氨酸和苯乙酸的混合物。该混合物用c(HC l)=6 mol/L的盐酸高温回流6 h得D-丙氨酸盐酸盐,再经717阴离子树脂处理得D-丙氨酸。光学纯度为95.3%,总收率为72.5%。
N-Phenylacetyl-DL-alanine （ Ⅰ ） was formed by reacting DL-alanine and phenylacetyl choloride in molar ratio 1.1 ： 1 at pH = 9.5. Then ,by enzymatic hydrolyzation for 6 h at 37 ℃ and pH =7.5, Ⅰ produced a mixture of N-phenylacetyl-D-alanine and phenylacetic acid. The mixture was hydrolyzed by refluxing with HCl [c（HCl） =6 mol/L] for 6 h to produce D-alanine hydrochloride, which can be turned into D-alanine by treating with 717 anion resin. The optical purity of the product D-alanine is 95.3% ,and the overall yield is 72.5%.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2008年第11期1066-1069,共4页
Fine Chemicals
基金
国家技术创新基金(02CJ-13-01-16)
Supported by the technology innovation foundation of China(02CJ-13-01-16)
