摘要
目的建立叶下珠愈伤组织培养方法。方法采用组织培养方法,比较不同外植体、蔗糖、植物生长物质及其配比对叶下珠愈伤组织诱导的影响。结果茎段的诱导率最高,为55.56%,叶片诱导不出愈伤组织。6-BA是叶下珠愈伤组织形成的主要影响因素,2,4-D、NAA、蔗糖次之。结论叶下珠愈伤组织诱导及继代的适宜培养基为MS+2,4-D0.5mg/L+6-BA1.0mg/L+NAA0.1mg/L+蔗糖10g/L。
Objective To establish culture method for Phyllanthus urinaria. Methods To study the possible effective factors of culture condition by comparing with different explants, sucrose, plant growth substance, and its ratio. Results The inductivity of stem was the highest about 55.56%, but callus of leaves could not be induced. 6-BA was the most important factor on callus induction, followed by 2,4-D NAA and sucrose. Conclusion The optimal medium to induce and subculture is MS＋2,4-D 0.5 mg/L＋ 6-BA 1.0 mg/L＋NAA 0.1 mg/Lq-sucrose 10 g/L.
出处
《中草药》
CAS
CSCD
北大核心
2008年第11期1723-1726,共4页
Chinese Traditional and Herbal Drugs
基金
国家农业成果转化基金项目(05EFN213400124)
淮北市重大专项(06085)
关键词
叶下珠
组织培养
愈伤组织
Phyllanthus urinaria L.
tissue-culture
callus
