摘要
目的观察32^P-玻璃微球（32^P-GMS）间质给药后体内生物学分布与代谢。方法120只小鼠分别经肝脏或肌肉注射32^P-GMS，每只（1．80±0．05）MBq／50μl,不同时间点处死取血及脏器计算每克组织百分注射剂量率（％ID／g）；12只大鼠肝脏、肌肉每只注射（18．0±0．5）MBq／0．5m1，收集排泄物测累积排泄率。结果小鼠肝脏组给药肝叶％ID／g0时最高为1．38，15min降至0．71，非给药肝叶峰值15min为0．52，4h之后左右肝叶无区别；肺组织计数率值1h内上升，肝肺累积分流率为37．9％。肌肉组注射点％ID／g0时为31．47，15min～8h为25．06—11．92（中值20．97），12h为8．70，24h～14d为3．54—2．02（中值2．51），其他主要脏器放射性接近本底水平。大鼠肝脏组粪便和尿液14d累积排泄率分别为0．0751％和0．0586％；肌肉组分别为0．0401％和0．0385％。结论32^P—GMS间质注射适用于除肝脏以外的体内组织脏器恶性实体瘤的治疗。
Objective To observe the biodistribution and the metabolism of phosphorus-32 glass mierospheres （^32P-GMS） through interstitial administration. Methods Thirty-two P-GMS were injected into liver and muscle of 120 mice respectively by means of interstitial administration with dose of （ 1.80 ± 0.05 ） MBq/50μl. All of the mice were killed at different time points, blood was taken out and major organs were dissected,and the injection dosage rate （% ID/g） was measured. The stool and urine were collected after interstitial injection both in liver and muscle of 12 rats with the dose of （18.0 ± 0.5 ） MBq/ 0.5 ml,then the accumulative radioactivity counts percentage was measured. Results In liver injection group,the highest point of % ID/g was 1.38 at start time after administration, and decreased to 0.71 at 15 min. The % ID/g value at 15 min was 0.52 in none injected liver lobes ,and there was the similar tendency between left and right liver lobes after 4 h. The radioactivity in lung was increased obviously in 1 h, and the liver-lung accumulate split stream rate was 37.9%. In muscle injection group, the highest % ID/g at start time point was 31.47 after administration,25.06 to 11.92 in 15 min to 8 h （ midvalue was 20.97） ,8.70 at 12 h,and 3.54 to 2.02 in 24 h to 14 days （midvalue was 2.51 ）. The radioactivity of other organs and tissues was close to the background level. The accumulative radioactivity counts percentage of stool and urine was 0.0751% and 0. 0586 % respectively in rat liver group, and 0. 0401% and 0. 0385 % respectively in rat muscle group. Conclusion ^32P-GMS interstitial administration may have great potential to treat the malignancy solid tumor in all organs and tissues except liver.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第11期1430-1431,共2页
Chinese Journal of Experimental Surgery
基金
国家高技术研究发展计划“863”计划资助项目（2007-AA02Z471）
关键词
32^P
间质注射
生物学
代谢
Phosphorus-32
Interstitial injection
Biology
Metabolism
