摘要
目的探讨大鼠肾脏缺血再灌注损伤后骨形态发生蛋白-7(BMP-7)和转化生长因子-β1(TGF-β1)表达的发生机制和内在联系,为防治缺血再灌注损伤提供理论基础.方法建立大鼠肾脏缺血再灌注损伤模型,随机分为实验组和假手术组,采用免疫组化(SABC法)检测各组大鼠左肾组织中BMP-7与TGF-β1蛋白的表达水平,分析两种因子表达的变化趋势,进行比较和相关性分析.结果实验组与假手术组相比,再灌注2、12、24h后的肾组织中BMP-7蛋白的表达随着缺血再灌注损伤的加重而逐渐减弱,再灌注损伤48、72h后表达逐渐恢复;再灌注2、12、24h后的肾组织中TGF-β1蛋白的表达随着损伤的逐渐加重而增强,再灌注损伤48、72h后表达逐渐下降;BMP-7与TGF-β1蛋白的表达在统计学上有明显的负相关性.结论在肾脏缺血再灌注损伤后BMP-7随着损伤的加重表达逐渐减弱,而TGF-β1却随着损伤的加重表达增强,两者的变化趋势一致,统计学上有明显的负相关性,可能在缺血再灌注的损伤和恢复过程中两者是一对重要的相关因子.
Objective To investigate the expression of BMP-7 and TGF-β1 in kidney following ischemia -reperfusion injury in rat and the relationship of them in the injury induced by ischemia-repersion. Methods The ischemia-reperfusion injury model in rat was established with its right kidney excised and left renal artey blocked for 60 minutes. 60 SD rats were devided into sham group and experiment group. The renal tissue was observed by optical microscope and the expression of BMP-7 and TGF-β 1 were observed by immunohistochemical stain. The expression of BMP-7 and TGF- β 1 were measured by image analysis system. Results The expression of BMP-7 decreased makedly at 24 h after reperfusion in experiment group compared with sham group, the return started from 48 h. The expression of TGF- β 1 increased from 2 h to 24 h after reperfusion in experiment group than sham group, the slowly return started from 48 h. The expression of BMP-7 was negatively related to TGF-β 1. Conclusions The expression of BMP-7 will downregulate with the aggravated damage following ischemia-reperfusion injury, while the expression of TGF- β 1 will upregulate with the aggravated damage following ischemia-reperfusion injury, They show negatively relationship in statistics.The BMP-7 and TGF- β 1 are a pair of related factors in kidney following ischemia-reperfusion injury.
出处
《昆明医学院学报》
2008年第5期56-59,共4页
Journal of Kunming Medical College
