摘要
目的红树植物秋茄(Kandelia candel)根提取物的体外抑菌和抗肿瘤活性筛选。方法采用微量稀释法和MTT法测定各提取部位的体外抑菌和抗肿瘤活性。结果秋茄根的石油醚提取物体外对金黄色葡萄球菌、表皮葡萄球菌、枯草芽胞杆菌和藤黄八叠球菌的最低抑菌浓度(MIC)分别是3.13、1.56、0.78和3.13mg.mL-1,而乙酸乙酯和乙醇提取物体外对表皮葡萄球菌的MIC分别为3.13和6.25 mg.mL-1;此外,石油醚提取物体外对人肝癌细胞SMMC-7721、小鼠黑色素瘤细胞B16、人胃癌细胞BGC803和肝癌细胞株HepG2的半数抑制浓度(IC50)分别为355.2、378.6、275.6和411.6μg.mL-1,而其它提取物则对肿瘤细胞的增殖具有明显的刺激作用。结论秋茄根的各有机溶剂提取成分分别具有不同的生物活性,具有潜在的药用开发价值。
Objective To investigate the antimicrobial and antitumor activities of extracts from the root of mangrove plant, Kandelia candel. Methods Micro-dilution and MTT method were used to test the antimicrobial and antitumor activities of extracts from the root of K. candel. Results The petroleum ether extracts of root could inhibit the growth of Staphylo- coccus aureus , S. epiderrnidis, Bacillus subtilis and Sarcina lutea with the minimal inhibitory concentration（MIC） of 3. 125 mg·mL^-1, 1. 5625 mg·mL^-1 , 0. 78125 mg · mL^-1 and 3. 125 mg·mL^-1 respectively. The ethyl acetate extracts and ethyl alcohol extracts could inhibit the growth of S. epiderrnidis with MIC of 3.13 mg·mL^-1 and 6.25 mg·mL^-1 respectively. At the same time, the petroleum ether extracts could inhibit the proliferation of tumor cells, such as SMMC-7721, B16, BGC803 and HepG2 with IC50 of 355.2μg·mL^-1, 378.6μg· mL^-1 , 275.6μg·mL^-1 and 411.6μg·mL^-1 respectively, while the other extracts could stimulate the proliferation of tumor cells apparently. Conclusion The antimicrobial and antitumor activities of extracts from the root of K. candel indicate the potential pharmaceutical usage of this plant.
出处
《中国海洋药物》
CAS
CSCD
2008年第5期15-17,共3页
Chinese Journal of Marine Drugs
基金
教育部高等学校科技创新工程重大项目培育资金项目(No.130105)
关键词
红树植物
秋茄
提取物
抑菌
抗肿瘤
mangrove
Kandelia candel
extracts
antimicrobial
antitumor
