摘要
目的探讨肝细胞生长因子（HGF）在颅脑创伤后的表达趋势，为硕脑创伤治疗中的HGF干预策略提供前期研究基础。方法96只wistar大鼠按随机数字表法分为实验组和假手术组，实验组为液压冲击中度颅脑创伤大鼠，并分为伤后2h、6h、12h、24h、72h、168h、336h组，假手术组不致伤，每组再分为两个亚组．每亚组6只，一组行HE及免疫组化染色，观察伤后病理变化及HGF的表达部位和表达量，另外一组用RT—PCR的方法观察创伤后HGFmRNA表达情况。结果在创伤后的大鼠大脑皮层组织中，HGF在蛋白水平以及基因水平都出现表达增高的情况。创伤边缘区HGF阳性细胞数从伤后24h开始增多，168h达高峰，336h有所下降，但仍高于伤前水平，差异有统计学意义（P〈0．05）。HGFmRNA表达量从创伤后72h开始增加，168h达高峰，与假手术组比较差异有统计学意义（P〈0．05）。结论HGF作为神经营养因子和血管生长因子，可能参与了颅脑创伤后神经元的保护和组织的修复、再生。
Objective To study the temporal expression pattern of hepatocyte growth factor （HGF） in the brain tissue of rats alter traumatic brain injury （TB1） and provide evidence for HGF-targeted interventions for TBI. Methods Ninety-six Wistar rats were randomly divided into 8 groups including a sham-operated group and 7 TBI groups （n=12） examined at 2, 6, 12, 24, 72, 168 and 336 h after to fluid percussion injury of the brain. Each group was randomized into two subgroups （n=6）, one for examining HGF expression and neuropathological changes in the brain with immunohistochemistry and HE staining, and the other for detecting the expression of HGF mRNA using RT-PCR. Results HGF expression increased at both the protein and mRNA levels after TBI. The number of HGF-positive cells began to increase on the margin of the injured area 24 h after the injury till reaching the peak level at 168 h. At 336 hatter the injury, the number of HGF-positive cells declined but still maintained a high level. The expression of HGF mRNA began to increase 72 h after injury and reached the peak level at 168 h. Conclusion As a neurotrophic factor and an angiogenic factor, HGF may contribute to neuronal protection and nerve tissue repair and regeneration after TBI.
出处
《中华神经医学杂志》
CAS
CSCD
2008年第11期1138-1141,共4页
Chinese Journal of Neuromedicine
关键词
肝细胞生长因子
颅脑损伤
Hepatocyte growth factor
Traumatic brain injury
