摘要
目的:探讨葛根素对肝缺血再灌注损伤Caspase-3、Bcl-2表达及肝细胞凋亡的影响。方法:建立大鼠肝脏部分缺血再灌注模型,以葛根素预处理,免疫组化法检测Caspase-3和Bcl-2表达,透射电镜观察肝细胞凋亡的超微结构改变。结果:肝缺血再灌注后发生了显著的细胞凋亡现象,Caspase-3表达升高,Bcl-2蛋白表达减少,6h恢复正常,其后逐渐升高。葛根素预处理使细胞凋亡明显减轻,Caspase-3表达明显下降,Bcl-2持续高表达。结论:葛根素可上调Bcl-2的表达,抑制Caspase-3的表达,减少肝细胞凋亡,对肝脏缺血再灌注损伤有保护作用。
Objective ：To investigate the effect of puerarin on the expression of Caspase-3 and Bcl-2 and the apoptosis of hepato- eytes in rat＇s hepatic ischemia reperfusion injury.Methods：A rat model of partial hepatic ischemia-reperfusion was found. The Cas- pase-3 and Bcl-2 expression were measured with immunohisto- chemical method. The apoptosis of hepatocytes were observed under electron microscope.Results：Universal hepatic cell apoptosis was found after reperfusion. The expression of Caspase-3 increased significantly, and the expression of Bcl-2 was lower at 3h after reperfusion, returned to normal level at 6h and continued to increase until 48h. When treated with puerarin, there was much less apoptosis, the expression of Caspase-3 decreased obviously and the positive Bcl-2 was significantly higher.Conclusions：Puerarin can protect the liver from the ischemia reperfusion injury by increasing Bcl-2 expression and inhibiting Caspase-3 expression, alleviating the apoptosis of hepatocytes.
出处
《交通医学》
2008年第5期472-473,共2页
Medical Journal of Communications
