摘要
目的寻求一种以B群链球菌（group B Streptococcus，GBS）菌体免疫原性蛋白为基础的疫苗。方法以一步层析法从GBSⅢ型菌32325株中获得42KD菌体蛋白，以42KD蛋白不同剂量和针次免疫NIH成年小鼠，间接ELISA法检测小鼠血清IgG抗体滴度。选择最佳免疫条件免疫小鼠，然后腹腔攻击GBSⅢ型32325菌株，观察主动免疫和被动免疫保护效果。结果目的蛋白的相对分子质量（Mr）为42．33×10^3，等电点为6．6，并初步鉴定为糖蛋白且为分泌性蛋白质。不同剂量免疫均能诱导小鼠产生相应IgG抗体，其效价最高可达1：24576，说明42KD蛋白具有很好的免疫原性。试验组成年小鼠的免疫保护指数为100，乳鼠的免疫保护指数为40。结论42KD蛋白对成年小鼠和乳鼠均诱导保护免疫。不同剂量免疫保护试验结果说明：在一定范围内，免疫剂量与血清IgG抗体应答水平呈正相关；小鼠血清IgG抗体滴度与保护力正相关。
Objective To look for a vaccine on the base of surface protein which can elicit protective immunity. Methods Purification of 42KD protein from Streptococcus serotype Ⅲ was used one-step chromatography. Antibody response was evaluated in mice immunized by 42KD protein with different immunization dose and by the different immunization procedure. After immunizing the mice in the optimum procedure, we challenge the mice in intradermal with Streptococcus to observe the active and passive protection. Results The molecular weight （ Mr ） of the protein was 42.33 × 10^3 and its point isoelectric （pI） was 6.6. Its property might be a glycoprotein which could be released to supernatant. The highest titer of antibody against 42KD protein was 1 ： 24 576. Which indicated that 42KD protein had good immunogenieity. The immunity exponent of NIH mice was 100 in test group and infant mice was 40. Conclusion The data suggests that 42KD protein may elicit protective immunity to adult and infant mice. Through protecting test with different immunizing doses, the findings indicate that protecting capability is relative to the titer of IgG specific antibody.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2008年第10期928-933,共6页
Chinese Journal of Microbiology and Immunology
关键词
B群链球菌
结合疫苗
免疫
主动保护
被动保护
Group B Streptococcus
Conjugate vaccine
Immunity
Active protection
Passive protection
