摘要
目的观察PPARγ配体罗格列酮(ROS)和GW9662对3T3-L1脂肪细胞Visfatin mRNA表达的影响,探讨PPARγ与Visfatin的关系。方法以不同浓度ROS和GW9662于各时段处理3T3-L1脂肪细胞,用RT-PCR技术检测VisfatinmRNA的表达。结果1、10、100μmol/L ROS抑制3T3-L1脂肪细胞Visfatin mRNA表达。1、5、25μmol/L GW9662抑制3T3-L1脂肪细胞Visfatin mRNA表达。5μmol/L GW9662不能拮抗10μmol/L ROS对Visfatin mRNA的抑制作用。
Objective To observe the effects of PPARγ ligands rosiglitazone and GW9662 on Visfatin mRNA expression in 3T3-L1 adipocytes.Methods Rosiglitazone and GW9662 in different concentrations were added into the culture medium of differentiated adipocytes for various times.Visfatin mRNA expression was measured by semiquantitative RT-PCR technique.Results ① 1,10,100 μmol/L rosiglitazone suppressed the Visfatin mRNA expression in 3T3-L1 adipocytes.② 1,5,25 μmol/L GW9662 suppressed the Visfatin mRNA expression in 3T3-L1 adipocytes.③ 5 μmol/L GW9662 could not antagonize the suppression of 10 μmol/L rosiglitazone on Visfatin mRNA expression in 3T3-L1 adipocytes.Conclusion rosiglitazone may affect the Visfatin mRNA expression via PPARγ-independent pathways.
出处
《安徽医科大学学报》
CAS
北大核心
2008年第5期504-508,共5页
Acta Universitatis Medicinalis Anhui
