摘要
目的评价舒芬太尼预先给药对大鼠心肌缺血再灌注损伤的影响。方法健康雄性SD大鼠36只，体重250～350g，采用结扎左冠状动脉前降支法制备心肌缺血再灌注模型。随机分为6组（n=6），假手术组（S组）只穿线，不结扎左冠状动脉前降支；缺血再灌注组（I／R组）缺血30min，再灌注120min；缺血预处理组（IPC组）缺血5min，再灌注5min，重复3次后缺血30min，再灌注120min；15组、MS组和HS组分别静脉输注舒芬太尼0．05、0．2、1．0μg·kg^-1·min^-1，持续5min，停5min，重复3次后缺血30min，再灌注120min。于缺血前即刻（T0）、缺血30min（T1）、再灌注30min（T2）、60min（T3）和120min（T4）时记录心率（HR）、左心室收缩峰压（LVSP）、左心室舒张末期压力（LVEDP）和左心室内压上升／下降最大速率（±dp／dtmax），计算左心室发展压（LVDP）。于T2时行心律失常严重程度评分；于T4时处死大鼠，取股动脉血样3ml，采用硫代巴比妥酸（TBA）法测定血清丙二醛（MDA）浓度，黄嘌呤氧化酶法测定血清超氧化物歧化酶（SOD）活性，计算心肌梗塞区（IS）面积与缺血危险区（AAR）面积的比值（IS／AAR），以IS／AAR表示心肌损伤程度。结果与S组比较，其余5组SOD活性降低，I／R组MDA浓度升高，IPC组及HS组MDA浓度降低（P〈0．05或0．01）；与I／R组比较，IPC组、LS组、MS组和HS组心律失常严重程度评分、IS／AAR及MDA浓度降低，IPC组SOD活性升高（P〈0．05或0．01）；与IPC组比较，LS组心律失常严重程度评分及IS／AAR升高（P〈0．05或0．01）。结论舒芬太尼预先给药可减轻大鼠心肌缺血再灌注损伤，与剂量有关。
Objective To investigate the effects of sufentanil pretreatment（SPC） on myocardial ischemiareperfusion （I/R） injury in rats. Methods Thirty-six male SD rats weighing 250-350 g were randomized into 6 groups （n = 6 each） ： group Ⅰ sham operation （S） ; group Ⅱ I/R; group Ⅲ isehemic pretreatment ＋ I/R （IPC） ; groupⅣ, Ⅴ, Ⅵ SPC （0.05, 0.2 and 1.0 μg·kg^-1·min^-1） ＋ I/R （SPC1,2,3）. The animals were anesthetized with pentobarbital sodium 60 mg/kg intraperitoneally. The left anterior descending branch of coronary artery was temporarily ligated for 30 min followed by 120 min reperfusion. In group Ⅲ （IPC） the animals were subjected to 3 episodes of 5 rain myocardial ischemia at 5 rain intervals before I/R. In group Ⅳ , Ⅴ and Ⅵ （SPC1, 2,3） the animals received 3 episodes of 5 min sufentanil infusion at 0.05, 0.2 and 1.0 μg·kg^-1·min^-1 respectively at 5 min intervals before I/R. HR, LVSP, LVEDP and ± dp/dtmax, were measured and left ventrieular developed pressure （LVDP） was calculated immediately before （T0 ）, at the end of 30 min ischemia （T1 ） and at 30 （T2） 60 （T3 ） and 120 rain of reperfusion （T4） . The severity of arrhythmia was assessed at T2 . The animals were killed at T4 . Plasma MDA concentration and SOD activity were determined. The infarct size （IS） and the area at risk （AAR） were measured by TIC staining. Results The plasma SOD activity was significantly decreased in groupⅡ-Ⅵ as compared with group S. The plasma MDA concentration was significantly increased by I/R in group Ⅱ as compared with group S. IPC and SPC attenuated the I/R-induced increase in plasma MDA concentration in group Ⅲ, Ⅳ, Ⅴ and Ⅵ . The arrhythmia score and IS/AAR were significantly lower in group IPC and SPC1,2,3 than in group I/R. Conclusion Sufentanil pretreatment can protect myocardium against I/R injury at the dose of 0.05,0.2 and 1.0 μg·kg^- 1 ·min^- 1 in rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2008年第10期932-935,共4页
Chinese Journal of Anesthesiology
