摘要
采用反相高效液相色谱法，进行了氨茶碱血药浓度的快速测定方法的研究。结果表明，将血样经甲醇沉淀后，直接进样，在色谱柱为ＯＤＳ－Ｃ１８柱，流动相为０．０２ｍｏｌ／ＬＫ２ＨＰＯ４甲醇水（３５：６５，ｐＨ６．９０），检测波长为２７０ｎｍ的色谱条件下，以可可碱为内标，氨茶碱的血药浓度的最低检出浓度为５０μｇ／Ｌ，线性范围为０．５～２０ｍｇ／Ｌ，相关系数ｒ＝０．９９９７。为临床氨茶碱血药浓度的快速测定提供了新的方法。
Rapid determination of the level of aminophlline in serum was studied by reversed phase high performance liquid chromatgraph.The serum was precipitated by methanol and then filtred.The solution was injected into ODS-C 18 column.Aminophlline was detected at 270 nm,using 0.02 mol/L K 2HPO 4 methanol-water(35:65,pH 6.90) as mobile phase.The minimum working concentration was 50 μg/L,the standard curve was linear over the concentration range from 0.5 to 20 mg/L ( r =0.9997).
出处
《武警医学》
CAS
1997年第1期15-16,共2页
Medical Journal of the Chinese People's Armed Police Force
关键词
高效液相色谱法
氨茶碱
血药浓度
High performance liquid chromatgraphy Aminophlline Blood concentration
