摘要
目的:探讨特异性环氧化酶-2(COX-2)抑制剂塞来昔布(celecoxib)对人神经母细胞瘤细胞系SK-N-SH细胞生长的影响及其分子生物学作用机制。方法:不同浓度塞来昔布(12.5、25、50和75μmol/L)用不同时间(24、48和72h)处理SK-N-SH细胞,MTT法检测细胞增殖,DNA ladder法及AO/EB染色法分析细胞凋亡,Western blot检测COX-2、Bcl-2蛋白表达。结果:MTT法显示,12.5、25、50和75μmol/L组在3个时间点对细胞的抑制率分别为(7.38±1.12)%、(10.33±1.97)%和(25.16±5.58)%;(34.46±6.76)%、(30.12±6.71)%和(57.54±3.06)%;(61.85±4.01)%、(50.78±2.85)%和(85.67±2.17)%;(83.85±5.56)%、(90.06±5.71)%和(98.04±4.43)%。组间差异均有统计学意义,P<0.05。结论:塞来昔布抑制SK-N-SH细胞的生长并诱导其凋亡,其机制除了抑制COX-2外还可能与抑制Bcl-2有关,有一定的临床应用价值。
OBJECTIVE：To investigate the influence of high selective cyclooxygenase-2（COX-2） inhibitor celecoxib on proliferation and apoptosis of SK-N-SH cells and reveal the potential COX-2-independent mechanism. METHODS： After SK-N-SH cells were treated with celecoxib in different doses （12.5, 25, 50 and 75 μmol/L） and different time （24, 48 and 72 hours）. The methabenzthiazuron （MTT） assay was used to measure the proliferation. The DNA ladder and AO/EB staining were used to observe the apoptosis. The expressions of COX-2 and Bcl-2 were detected by Western blot before and after the treatment with celecoxib. RESULTS： From MTT, the inhibitory rates of celecxib on SK-N-SH cells in different dose groups at three different time were （7.38±1.12）%,（10.33±1.97）%,（25.16±5.58）%, （34.46±6.76）%,（30.12±6.71）%,（57.54±3.06）%, （61.85±4.01）%,（50.78±2.85）%,（85.67±2.17）%, （83.85±5.56）%,（90.06±5.71）%,（98.04±4.43）%, respectively, and there were significant difference among each groups, P〈0.05. CONCLUSIONS： Celecoxib inhibits the growth and induces apoptosis of SK-N-SH cells through down-regulating the expressions of COX-2 and Bcl-2 in vitro. The COX-2-independent effect may exist. This provides a new therapeutic option for neuroblastoma patients.
出处
《中华肿瘤防治杂志》
CAS
2008年第16期1218-1221,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
教育部国家留学基金(2002)
关键词
神经母细胞瘤
细胞增殖
细胞凋亡
neuroblastoma, cell proliferation, cell apoptosis
