摘要
德氏乳杆菌保加利亚亚种等pH值培养的适宜pH值为6.0。对单一补料分批培养进行理论分析,在准恒定状态下,稀释率与比生长速率基本相同,即μ≈D。采用全营养物料连续流加法,在对数生长末期进行补料操作,确定德氏乳杆菌保加利亚亚种起始流加速率为5.874×10-4m3/h。补料培养的德氏乳杆菌保加利亚亚种的菌体密度可达1.0×1010cfu/mL。
The optimum pH value for LDB （Lac. delbrueckii ssp. bulgaricus） growth was 6.0 during constant pH value cultivation. The single nutritious materials supplement fed cultivation was analyzed theoretically, dilute rate was about equal to specific growth rate, i.e. μ≈D under the quasi-constant state. The determined initial supplement method was 0.5874 The density of LDB was up to material supplement rate using whole nutritous materials continuous L/h at the end of logarithmic growth period in the incubation of LDB. 1.0×10^10 cfu/mL under the conditions.
出处
《乳业科学与技术》
2008年第6期263-265,共3页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金
国家"十一五"科技支撑计划(No.2006BAD04A14)
国家高技术研究发展计划(863计划)(No.2007AA10Z353)
关键词
德氏乳杆菌保加利亚亚种
等pH值培养
补料分批培养
LDB （Lac. delbrueckii ssp. bulgaricus）
constant pH value cultivation
nutritious materials supplement fed cultivation
