摘要
目的研究异基因造血干细胞移植(Allo-HSCT)后患者CD4+CD25+调节性T细胞(CD4+CD25+Treg)与急性移植物抗宿主病(aGVHD)、慢性移植物抗宿主病(cGVHD)的关系。方法采用流式细胞术检测50例Allo-HSCT后患者及24例健康者外周血CD4+CD25+Treg在CD4+T细胞中的百分含量。结果50例患者均获得造血功能重建。正常对照组与无移植物抗宿主病(GVHD)组CD4+CD25高度敏感(CD4+CD25high)、CD4+CD25+CD127低度敏感(CD4+CD25+CD127low)T细胞百分比分别为(2.185±0.582)%和(6.365±2.155)%vs(2.320±2.813)%和(7.515±5.457)%(P>0.05)。aGVHD组为(1.270±1.385)%和(4.575±3.900)%,低于正常对照组与无GVHD组(均P<0.01);其中,Ⅱ~Ⅳ度aGVHD组显著低于Ⅰ度aGVHD组(P<0.01)。cGVHD组为(1.375±0.812)%和(5.620±1.770)%,低于无GVHD组(P<0.01);其中,广泛性cGVHD组低于局限性cGVHD组(P<0.05)。结论CD4+CD25+Treg的水平与Allo-HSCT后GVHD的发生及严重程度有密切关系;通过监测CD4+CD25+Treg水平对临床早期诊断GVHD及判断GVHD预后、指导免疫调节剂的应用具有重要意义。
Objective To investigate the relationship between CD4^＋ CD25^＋ regulatory T cells （CD4^＋ CD25^＋ T reg） and acute graft-versus-host disease（aGVHD） or chronic GVHD（cGVHD） after allogeneic haematogenesis stem cell transplantation （Allo-HSCT）. Methods The percentage of CD4^＋ CD25^＋ T reg of peripherial blood in CD4^＋ T cells in 50 patients with hematological malignancies after Allo-HSCT and 24 healthy people were detected by flow cytometry. Results All the patients achieved engraftment. The percentage of CD4^＋ CD25^high ,CD4^＋ CD25^＋ CD127^low T in the groups of normal control and those without GVHD were （2. 185 ± 0. 582） %and （6. 365 ± 2. 155） %; （2. 320 ± 2. 813） % and （7. 515±5. 457） respectively（ P 〉0.05） ;The percentage of CD4^＋ CD25^high ,CD4^＋ CD25^＋ CD127^low T in the groups of aGVHD were （1.270 ± 1. 385） %and （4.575 ± 3. 900） %, declined significantly compared with those of normal control and without GVHD （all P 〈0. 01）. The percentage of CD4^＋ CD25^＋ T reg in the peripheral blood of patients with Ⅱ-Ⅳ aGVHD declined significantly compared with I aGVHD（ P 〈0.01） and there was significant difference between extensive cGVHD and limited cGVHD（ P 〈0.05）. Conclusion CD4^＋ CD25^＋ T reg cell level is relative to aGVHD or cGVHD. It is very significant in clinic to detect CD4^＋ CD25^＋ T reg after Allo-HSCT.
出处
《临床荟萃》
CAS
北大核心
2008年第21期1524-1527,共4页
Clinical Focus
基金
国家自然科学基金(30771954)
