摘要
目的:研究青蒿素对肿瘤细胞DNA的损伤作用。方法:体外培养人白血病K562细胞,MTT法测定IC50,应用单细胞凝胶电泳(SCGE)检测细胞核DNA的损伤。结果:青蒿素处理K562细胞IC50为1.5×10-5mol/L,1×10-5mol/L青蒿素处理细胞24h后可引起细胞核DNA的损伤。结论:青蒿素造成肿瘤细胞DNA的损伤,抑制肿瘤细胞生长。
Objective：To study the damage to tumor cell DNA by artemisinin. Methods：The K562 cell DNA damage was studied by MTT,and single ceil gel electrophoresis. Results：We added different concentrations of artemisinin into the medim in which the cells were cultured,and the IC50 values were 1.5 ×10^-5mol/L and 1 ×10^-5mol/L. Single cell gel electrophoresis showed a comet-like tail. Conclusion：Artemisinin can damage DNA and inhabit proliferation of K562 cells.
出处
《黑龙江医药科学》
2008年第5期15-16,共2页
Heilongjiang Medicine and Pharmacy
基金
天津市科委课题资助(No.05YFJMJC08200)
武警医学院院级项目(MY2005-6)
关键词
青蒿素
单细胞凝胶电泳
DNA损伤
artemisinin
single-- cell gel electrophoresis
DNA damage
