摘要
目的探讨cathepsin L蛋白在肝细胞癌中的表达及其与肿瘤临床病理特征的关系。方法58例肝细胞癌中病理分级高分化18例、中分化18例、低分化22例;临床分期Ⅰ+Ⅱ期26例、Ⅲ+Ⅳ期32例。采用免疫组织化学染色、RT-PCR法检测cathepsin L表达,结合患者临床病理指标及预后进行分析。结果cathepsin L表达阳性率为72.4%(42/58),不同病理分级、临床分期及转移分组cathepsin L表达阳性率差异显著。cathepsin L mRNA检出阳性率65.5%(38/58),cathepsin L mRNA的表达与肝细胞癌的病理分级、临床分期及淋巴转移有相关性。结论cathepsin L在肝细胞癌的生长浸润、侵袭转移中发挥重要作用,可作为肝细胞癌预后判断的客观指标之一。
Objective To explore the expression of cathepsin L in hepatocellular carcinoma and to analyze its relationship with clinical and pathological features. Methods Fifty-eight specimens of hepatocellular carcinoma were studied, including 18 well-differentiated, 18 moderately differentiated and 22 poorly differentiated. Classified by TNM clinical stage, 26 were in Ⅰ / Ⅱ stage and 32 in Ⅲ/Ⅳ stage. The expressions of cathepsin L were detected with immunohistochemistry and RT-PCR. Their relationship with the clinical and pathological features of hepatocellular carcinoma was analyzed. Results The positive rate of cathepsin L protein was 72.4% （42/58） , and the expressions of cathepsin L protein were significantly different among the specimens of pathological grades of tumor, of TNM clinical stages and with/without metastasis. The positive rate of cathepsin L mRNA was 65.5% （38/58）. The expression of cathepsin L mRNA in hepatocellular carcinoma was correlated with pathological grades of tumor, TNM clinical stages and lymph node metastasis. Conclusion Cathepsin L plays an important role in the occurrence of hepatocellular carcinoma for its correlation with infiltration and metastasis of hepatocellular carcinoma. Cathepsin L can be used as a useful marker for the prognosis of hepatocellular carcinoma patients.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2008年第22期2139-2141,共3页
Journal of Third Military Medical University
