摘要
目的了解乙型肝炎患者前S1抗原（Pre-S1）、HBVDNA及HBe舷的相关性，并评价它们在诊断HBV复制中的临床意义。方法应用ELISA、荧光定量PCR法（FQ-PCR）对198例乙型肝炎患者进行Pre-S1、HBVDNA及HBV血清标志物五项（两对半）检测。结果198例乙型肝炎患者中Pre-S1阳性107例（54．0％），HBVDNA阳性111例（56．1％），HBeAg阳性90例（45．5％），三者同时阳性72例（36．4％），Pre-S1与HBVDNA阳性率比较，差异无统计学意义（P〉0．05）。在90例HBeAg阳性样本中，Pre-S1阳性72例（80．0％），HBVDNA阳性74例（82．2％），三者具有较高的符合率。在108例HBeAg阴性样本中，Pre-S1阳性35例（32．4％），HBVDNA阳性37例（34．2％），Pre-S1与HBVDNA检出率比较，两者高度一致（χ^2=0．08，P〉0．05）。结论Pre-S1与HBVDNA能敏感反映HBV复制的情况，尤其可以协助诊断HBeAg阴性的乙型肝炎患者是否有HBV复制，对于判断患者的病情及指导治疗具有重要的临床意义。
Objective To study the associations among Pre-S1 antigen（Pre-S1）, HBV DNA and HBeAg and explore the significance of HBV Pre-S1, HBV DNA, HBeAg detection in the viral replication. Method Pre-S1, serum markers of HBV and HBV DNA concentrations of 198 sera of hepatitis B patients were detected by ELISA and FQ-PCR. Results The positive rates of Pre-S1, HBV DNA and HBeAg were 54.0% （107/198）, 56.1% （111/198） and 45.5%（90/198） respectively among 198 patients . The same positive rates of them were 36.4% （72/198）. In 90 patients with HBeAg positive, the positive rates of Pre-S1 and HBV DNA were 80% （72/90）,82.2% （74/171） respec- tively, the coincidence rates of them were great （P 〉 0.05）. In 108 patients with HBeAg negative, the positive rates of Pre-S1 and HBV DNA were 32.4% （35/108）, 34.2% （37/108） respectively. There was no significant difference in positive rate between Pre-S1 and HBV DNA（ χ^2 = 0.08, P 〉 0.05）. Conclusions Pre-S1 and HBV DNA can reflect the replication of HBV sensitively, especially for those who are HBeAg negative, and can be used as a helpful tool for the diagnosis of HBV infection and clinical evaluation.
出处
《国际流行病学传染病学杂志》
CAS
2008年第5期295-297,共3页
International Journal of Epidemiology and Infectious Disease
关键词
肝炎
乙型
前S1抗原
病毒
复制
Hepatitis B
Pre-S1 antigen
Virus
Replication
