摘要
Genetic diversity and differentiation of the oyster Crassostrea plicatula populations from China’s coast were studied based on seven microsatellite loci. All loci showed high polymorphism for all five C. plicatula populations,with an average number of allele per locus of 19.3-27.9 and an average expected heterozygosity of 0.889-0.952. Significant departures from Hardy-Weinberg equilib-rium and deficits of heterozygotes were observed over most populations at each locus,which were fully explained by null alleles. Microsatellite analysis revealed significant subdivision in the C. plicatula populations. According to the neighbor-joining tree con-structed on the basis of the DA distance,the five populations fell into three regional groups,showing a relatively homogeneous genetic structure in geographically close populations. Assignation tests correctly assigned high percentages of individuals to their original populations and groups,and also confirmed the existence of genetic differentiation among C. plicatula populations. The results ob-tained in this study will facilitate the formulation of appropriate fisheries management programs,stock identification and conservation of biodiversity for the species.
Genetic diversity and differentiation of the oyster Crassostreaplicatula populations from China＇s coast were studied based on seven microsatellite loci. All loci showed high polymorphism for all five C plicatula populations, with an average number of allele perlocus of 19.3-27.9 and an average expected heterozygosity of 0.889-0.952. Significant departures from Hardy-Weinberg equilibrium and deficits of heterozygotes were observed over most populations at each locus, which were fully explained by null alleles. Microsatellite analysis revealed significant subdivision in the C. plicatula populations. According to the neighbor-joining tree constructed on the basis of the DA distance, the five populations fell into three regional groups, showing a relatively homogeneous genetic structure in geographically close populations. Assignation tests correctly assigned high percentages of individuals to their original populations and groups, and also confirmed the existence of genetic differentiation among C. plicatula populations. The results obtained in this study will facilitate the formulation of appropriate fisheries management programs, stock identification and conservation of biodiversity for the species.
基金
grants from National High Technology Research and Development Program (2006AA10A409)
National Natural Science Foundation of China (No 30571442)
