摘要
以枇杷属(Eriobotrya Lindl)植物的若干个野生种为试材,进行种质资源离体保存方法(花粉超低温保存、种子(成熟胚)常温限制生长法和超低温保存、茎尖培养和叶片培养)的初步研究。主要结果如下:①不同枇杷野生种类间的花粉生活力存在差异;液氮冷冻后花粉生活力与对照相比无明显降低;经干燥的花粉生活力高于未干燥的,含水量低处理的花粉生活力强。②添加或不添加生长调节剂的mMS培养基均较有利野生枇杷种子(胚)的萌发和芽苗生长;不添加生长调节剂的B5培养基有利野生枇杷种子(胚)的胚根萌发,而胚芽和芽苗生长均慢,适合作为限制生长的种质离体保存的培养基。③经过液氮处理的普通枇杷野生种子(成熟胚)均未能成活。④台湾枇杷茎尖培养启动较容易,启动率为72%;怒江枇杷茎尖极易褐变,培养成活率极低;台湾枇杷叶片可在含2,4-D的MS培养基上诱导出米黄色愈伤组织,适宜的2,4-D浓度为0.2 mg·L^(-1)。
In order to set up in vitro preservation for wild loquat species germplasm （Eriobotrya）, several kinds of methods were carried out in this preliminary study. The tested materials and the treated methods included the pollen cryopreservation, preservation of seeds （ mature embryo） at regular temperature and cryopreservation, shoot - tip and leaf culture preservation. The results were as follow： 1 ） The pollen viability was different among different species; the pollen viability was no significant lower after cryopreservation; the dried pollen viability was higher than those not dry. 2） mMS medium with or without growth regulators all suited for embryo germination and shoot growth; while B5 medium suited for radicle growth but restrained growth, so B5 suited as the medium. 3 ） After the treat of cryopreservation with seeds （ mature embryos） was unable to survive. 4） the shoot tip of Eriobotrya deflexa Nakai was easier to start, generate rate was 72%, E. salwinensis Hand - Mazz was very easy for browning, the survival rate is extremely low ; the yellow callus could be induced from the leaves of Eriobotrya deflexa Nakai in the MS medium with 2, 4 - D and the appropriate concentration of 2, 4 - D was 0. 2 mg · L^-1.
出处
《福建果树》
2008年第3期28-32,共5页
Fujian Fruits
基金
广东省自然科学基金重点项目(07118121)
关键词
枇杷
野生种
种质离体保存
超低温保存
Eirobotrya
Wild species
Germplasm preservation in vitro
Cryopreservation
