摘要
【目的】研究通过视黄酸(RA)对大鼠急性放射性肺损伤的干预作用,阐明其对放射性肺损伤肺组织表皮生长因子受体(EGFR)表达的影响。【方法】健康雄性Wistar大鼠80只随机分为4组:正常对照组(A组)、单纯给药组(B组)、单纯照射组(C组)和照射加给药组(D组),每组20只。C、D两组大鼠行6MV-X线15Gy全胸野照射,B、D两组大鼠给予20mg/(kg·d)RA制剂灌服,于照射后第1、2、4、8周取右肺组织行HE和Masson染色,免疫组化方法检测EGFR的表达。【结果】HE和Masson染色提示照射后的1周始肺泡腔有炎性细胞渗出,继之间质水肿,4及8周出现肺泡腔变小甚至结构破坏,局部实变,肺间质出现胶原纤维;B组与A组比各时间段的病理变化无明显差别,但D组与C组比较大鼠肺炎和肺水肿减轻,肺组织胶原纤维量减少。EGFR免疫组化染色显示C组与A组相比在各时间段EGFR表达均明显增强(P<0.001),D组与C组比第4、8周EGFR表达明显减弱(P<0.001)。【结论】EGFR参与放射性肺损伤的发生、发展,而RA从蛋白质水平有效抑制EGFR表达,为治疗放射性肺损伤提供了实验依据。
[Objective] To investigate the intervention effect of retinoic acid （RA） on acute radiation-induced lung injury ,then to illuminate that RA effects the expression of epidermal growth factor reeeptor（EG-FR） in rat models . [Methods] Eighty Wistar rats were randomly divided into four groups with 20 rats in each： control group（ group A）,RA treatment group（group B）,radiation group（group C） and radiation combined with RA treatment group（group D）. Rats in group C and D were radiated with 6MV-X ray linear accelerator at a dose of 15Gy thoracically, and rats in group B and D were given RA by gastrogavage at a dose of 20mg/（ kg·d）, and rats in group A and C were given physiological saline at the same dose until they were killed. In 1, 2, 4 and 8 weeks, the pathological change of lung tissues and the expression of EGFR were detected with HE, Masson and immunohistochemistry staining, respectively. [Results] HE and Masson staining showed that only one week after radiation, the alveolar spaces had exudative inflammatory cells, following interstitial edema, and at 4 and 8 weeks, alveolar space shrank, even its structure was destroyed with local consol, and pulmonary interstitium appeared collagen fibers. There was no significant difference in pathological change be tween group A and B, but pneumonia and pulmonary edema in group D were obviously less than those in group C, and the expression of EGFR was significantly higher in group A and C at all times（P〈0.001） compared with control group, while it was lower in group D compared with group C at 4 and 8 week （P〈0. 001）. [Conclusion] EGFR may participate in the process of radiation induced lung injury, and RA could effectively inhibit the expression of EGFR in protein level.
出处
《医学临床研究》
CAS
2008年第10期1729-1732,共4页
Journal of Clinical Research
基金
辽宁省自然基金(20052080)资助
