摘要
目的研究醋酸曲谱瑞林对人子宫内膜癌细胞株HEC-1-B的作用及其分子作用机制。方法以不同浓度醋酸曲谱瑞林(0,10-9,10-8,10-7,10-6,10-5mol/L)体外作用于中分化人子宫内膜腺癌细胞HEC-1-B(0mol/L组作为对照组,其余各组作为实验组),用MTT法检测细胞抑制率;免疫细胞化学检测HEC-1-B细胞PCNA蛋白表达,并用病理图像分析软件进行半定量分析。RT-PCR法检测HEC-1-B细胞PCNAmRNA的表达。结果①当醋酸曲谱瑞林浓度为10-9mol/L时,HEC-1-B细胞即受到抑制,抑制率为36.8%;随着浓度的增大,抑制率渐高,到浓度为10-5mol/L时抑制率上升至57.4%。与对照组比较,实验组抑制率差异有统计学意义(P<0.05)。②浓度从10-9-10-5mol/L的醋酸曲谱瑞林作用72h后,PC-NA蛋白和PCNAmRNA表达均有不同程度的下降,与对照组相比差异有统计学意义(P<0.05)。结论醋酸曲谱瑞林在体外对HEC-1-B细胞可能有直接的抑制作用,且呈剂量依赖关系。醋酸曲谱瑞林抑制HEC-1-B细胞增殖的分子机制可能与下调PCNA蛋白表达有关。
Objective To explore the inhibitive effect of alarelin on cell proliferation of human endometrial cancer cell line HEC-1-B and its molecular mechanisms. Methods HEC-1-B cells were cultured in DMEM medium with alarelin and divided into six groups： experimental groups （ 10^-9,10^-8,10^-7,10^-6,10^-5 mol/L ） and control group （ 0 mol/L ）. The inhibitory rate of HEC- 1 -B cells was detected by MTT method. The expression of PCNA protein was detected by immunocytochemical method, and semi-quanti- tatively analyzed by pathological image analysis software. The expression of PCNA mRNA was detected by reverse transcription-poly- merase chain reaction. Results （1）The growth of HEC-1-B cells was inhibited at the concentration of 10^-9 mol/L, and the inhibitory rate was 36.8 %. The inhibitory rate increased in a dose-dependent manner. When alarelin concentration was 10^-5 mol/L, the inhibitory rate increased to 57.4 %. Compared with control group, the inhibitory rates were significantly higher in experimental groups （P 〈 0.05）. （2）Compared with control group, the expression of PCNA protein and PCNA mRNA significantly decreased after 72 h（P 〈 0.05）. Conclusion Alarelin might have direct inhibitory effect on HEC-1-B cells in vitro, which may be related to down-regulation of PCNA protein.
出处
《山西医科大学学报》
CAS
2008年第10期895-897,共3页
Journal of Shanxi Medical University
