摘要
目的观察重组人白细胞介素-6(rhIL-6)对SW872脂肪细胞蛋白质酪氨酸磷酸酶1B(PTP1B)、抵抗素及IL-6基因表达的影响,探讨rhIL-6对脂肪细胞分泌功能的调节作用。方法体外培养,油酸诱导SW872前脂肪细胞分化为成熟的脂肪细胞,在培养液中加入不同水平rhIL-6(0、1、5、10、20、50μg/L)作用24 h,加入20μg/L rhIL-6后分别作用不同时间(0、4、8、12、24 h)。收集细胞提取总RNA,采用半定量反转录酶聚合酶链反应方法检测SW872脂肪细胞PTP1B、抵抗素、IL-6 mRNA水平。结果rhIL-6 1μg/L作用24 h,对SW872脂肪细胞IL-6 mRNA的表达无影响;随着rhIL-6水平的增加,SW872脂肪细胞IL-6 mRNA的表达水平逐渐增加,但以20μg/L rhIL-6的作用最强(F=233.9 P<0.01);20μg/L rhIL-6作用4 h即可促进SW872脂肪细胞IL-6 mRNA的表达,随着作用时间的延长,其促进作用更加明显(F=247.8 P<0.01)。1μg/L rhIL-6作用24 h,对SW872脂肪细胞PTP1B mRNA的表达无影响;5μg/L rhIL-6即可促进SW872脂肪细胞PTP1B mRNA表达,50μg/L rhIL-6作用24 h,对PTP1B mRNA的表达促进作用更明显(F=515.58 P<0.01);20μg/L rhIL-6作用4 h对脂肪细胞PTP1B mRNA的表达无影响,作用8 h即可促进PTP1BmRNA的表达,随着作用时间的延长其作用更加明显(F=498.62 P<0.01)。不同水平、不同作用时间下,rhIL-6对SW872脂肪细胞抵抗素mRNA的表达无明显影响(F=9.6,10.5 Pa>0.05)。结论rhIL-6以剂量和时间相关的方式促进SW872脂肪细胞PTP1B及IL-6 mRNA表达,对抵抗素mRNA的表达无影响。
Objective To observe the effects of recombinated human interlcukin -6（ rhIL- 6） on expressions of protein tyrosine phosphatase - 1 B （ PTP1 B）, resistin and IL - 6 in SW872 fat cells, and evaluate the modulation function of rhIL - 6 on excretion function of adipocytes. Methods SW872 preadipocytes were induced to differentiate into mature adipocytes by oleic acid in vitro. After treatment with different concentrations of rhIL - 6 （0,1,5, 10,20,50 ug/L） for 24 h and 20 ug/L rhIL - 6 for different time （0,4,8,12,24 h）, Total RNA was isolated from adipocytes. The levels of PTP1B, resistin and IL- 6 mRNA expressions were measured by semiquantltative reverse transcriptase polymerase chain reaction assay. Results The cells treated for 24 h with rhIL -6 （ 1 ug/L） showed no change on IL -6 mRNA expression; however when the dose of rhIL -6 was increased upon 5 ug/L and more, the level of IL - 6 mRNA expression were elevated;but the level of IL - 6 mRNA expression were highest in cells treated for 24 h with 20 ug/L rhIL - 6 （ F = 233.9 P 〈 0.01 ） ; the level of IL - 6 mRNA expression of the cells treated for 4 h with rhIL -6（20 ug/L） were higher than that of control group ,with time gone ,the level of IL -6 mRNA expression increased furtherly（ F = 247.8 P 〈 0.01 ）. The cells incubated for 24 h with 1 ug/L rhIL - 6 showed no change of PTP1 B mRNA expression ;however, the level of PTP1 B mRNA increased with rising concentration of rhIL - 6 （ F = 515.58 P 〈 0.01 ） ; the cells treated for 4 h with 20 ug/L rhIL -6, the level of PTP1B mRNA did not increased; if the cells treated for 8 h, the level of PTP1B mRNA increased;with the time gone,the effect were stronger（ F =498.62 P 〈0. 01 ）. The cells treated with rhIL - 6（0, 1,5,10,20,50 ug/L, respectively） showed no changes on mRNA expression and incubated for different time（4,8,12 and 24 h） with 20 ug/L, rhIL-6 did not show significantly influences inhibition of resistin gene expression（ F = 9.6,10.5 Pa 〉 0.05 ）. Co
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2008年第20期1601-1603,共3页
Journal of Applied Clinical Pediatrics
