摘要
目的：建立蓬莪术药材醇提取物的LC—MS指纹图谱评价方法。方法：以四川中药材规范化种植基地的蓬莪术药材为研究对象，采用甲醇冷浸法提取蓬莪术有效成分，用液相色谱-质谱联用法测定其指纹图谱（LC-MS-FPS），并采用国家药典委员会颁布的中药色谱指纹图谱相似度评价系统软件（2004版）对测定结果进行评价。结果：四川种植的蓬莪术药材醇提取物的指纹图谱有良好的相似度，生成的指纹图谱，有14个共有特征峰。通过与对照品的保留时间、紫外光谱及LC-MS所得分子量信息比较，4～6号峰和9号峰分别鉴定为姜黄素、脱甲氧基姜黄素、莪术烯醇和莪术酮。结论：建立的蓬莪术醇提取物的LC-MS指纹图谱，为其质量控制提供科学依据。
Objective： To study and establish the fingerprint of Curcuma phaeocaulis by high performance liquid chromatography-mass spectrometry. Method： LC-MS analysis was carried out in a Hewlett Packard model 1100 liquid chromatograph coupled with electrospray mass spectrometry. The analysis was performed on a ZORBAX RX-C18 column （4. 6 mm × 250 mm, 5 μm） with a mobile phase of methanol-isopropanol-water （mathanoic acid 0. 4% ） in a gradient mode at a flow rate of 1.0 mL·min^-1, and the column temperature of 35 ℃. The wavelength of the UV detector was set at 254 nm. RSD values of precision, repeatability and stability of this LC-MS method were less than 5%. The total 10 batches of C. phaeocaulis from the different places of Sichuan province were detected, and different commodities and preparative methods were compared. Result： The standard fingerprint of the extract of C. phaeocaulis, which collected in the different places of Sichuan province, was originated from the Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004 software, and 15 common peaks existed in the fingerprint. Each peak in the fingerprint was separated well under the above analysis condition and similarities of the extracts of C. phaeocaulis samples of the ten batches were all above 0.90. By comparison of the retention time and the on-line UV spectra and their molecular weights of the chemical standards, peaks 4-6 and 9 were identified as curcumin （4）, demethoxycurcumin （5）, curcumenol （6）, and curcumone （9）, respectively. Conclusion： This LC-MS fingerprint and quantitative assessment can be used in the quality control of C. phaeocaulis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第19期2218-2221,共4页
China Journal of Chinese Materia Medica
