摘要
目的探讨落新妇甙对大鼠肺移植后机体急性排斥反应的影响和机制,以明确落新妇甙对大鼠肺移植急性排斥反应的作用。方法建立大鼠原位肺移植模型,术后将60只受体大鼠随机分为两组,对照组:术后用生理盐水1ml/d灌胃,实验组:术后用落新妇甙1ml/kg·d灌胃。观察肺移植后大鼠的存活时间、大鼠脾细胞T淋巴细胞转化率、脾淋巴细胞白细胞介素2(IL-2)的活性以及外周血中活化T淋巴细胞凋亡情况。在电子显微镜下观察肺血管超微结构变化。结果实验组大鼠肺移植后存活时间较对照组明显延长(25.4±2.1d vs.13.4±1.2d;t=2.042,P<0.05)。实验组脾细胞T淋巴细胞转化率较对照组明显降低(23465.8±8783.4cpm vs.74567.3±12874.6cpm;t=2.284,P<0.05);实验组移植大鼠脾淋巴细胞IL-2活性较对照组明显降低(4.25±2.65U/ml vs.23.46±1.82U/ml;t=3.165,P<0.01)。实验组能有效地诱导急性排斥反应中活化T淋巴细胞凋亡。实验组肺组织超微结构损伤较对照组减轻。结论落新妇甙通过下调IL-2产生,诱导活化T淋巴细胞凋亡,抑制T淋巴细胞增殖分化,广泛抑制了以T淋巴细胞为主的肺移植术后急性排斥反应,从而延长肺移植大鼠的存活时间。
Objective To investigate the suppression effect and mechanism of Astilbin on lung allograft rejection in rats, in order to know the function of Astilbin on rats＇ lung acute rejection. Methods The model of rat left lung transplantation was set up. Sixty lung transplanted rats were divided into two groups randomly, control group： rats were fed with normal saline Iml per day, experimental group： rats were fed with Astilbin 1mg/kg per day. Survival time, transforming rate of T cells in spleen, activity of interleukin 2 （IL-2） in spleen lymph cells and apoptosis of T cells were observed. Changes in uhrastructure of pulmonary arteries were observed by electron microscope. Results The survival time in experimental group was prolonged than that in control group （25.4± 2.1 d vs. 13.4±1.2 d;t=2. 042, P〈0.05）. Transforming rate of T cells of spleen in experimental group was significant lower than that in control group （23 465.8±8 783.4cpmvs. 74 567.3±12874.6cpm; t=2.284,P〈0.05）.Activity of IL-2 of spleen lymph cells in experimental group was significant lower than that in control group （4.25±2.65U/ml vs. 23.46±1.82U/mt; t=3. 165, P〈0.01）. Effectively derive apoptosis of activated T cells in acute rejection were observed in experimental group, the uhrastructure of pulmonary arteries showed attenuated injury in experimental group. Conclusion Astilbin decreased the IL- 2 concentration in plasma and induced the apoptosis in activated T cells, then suppressed the acute rejection of lung allograft and prolonged the survival period of lung transplantation rats.
出处
《中国胸心血管外科临床杂志》
CAS
2008年第5期365-368,共4页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
关键词
落新妇甙
肺移植
活化T细胞
Astilbin
Lung transplantation
Activate T cells
