摘要
目的:建立高效液相色谱法测定亚甲基蓝原料药的有关物质。方法:采用依利特C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为0.34%磷酸溶液(三乙胺调pH至3.0)-乙腈(77:23),流速1.0 mL·min^(-1),检测波长为246 nm。结果:有关物质与主药有较好的分离效果。结论:该方法简便灵敏、结果准确,可用于亚甲基蓝原料药的质量控制。
Objective ：To establish an HPLC method for the determination of related substances of methylene bLue. Method：Elite C18 column（250 mm ×4.6 mm,5 μm） was used,the mobile phase consisted of 0. 34% phosphoric acid （ adjusted to pH 3.0 with triethylamine） - acetonitrile （ 77 ： 23 ）, and the flow rate was 1.0 mL · min^ - 1. The detection wavelength was 246 nm. Result：Related substances and methylene blue could be separated well. Conclusion：The method is simple and accurate, and can be used for quality control of methylene blue.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第10期1698-1701,共4页
Chinese Journal of Pharmaceutical Analysis
