摘要
用人脐血造血细胞体外扩增后对受骨髓致死量照射的BALB／C小鼠进行移植。结果，移植组平均存活率为90％，对照组仅为10％。存活鼠骨髓和脾脏内可检测到CD45阳性的人源性造血细胞，体外祖细胞集落培养有人源性髓系和红系集落形成。结论，脐血造血细胞可帮助恢复急性重症放射损伤骨髓的造血重建。
To investigate alfernative source of hematoietic stem cells to rescue the sublethal radiation damage (SRD) casualties. Hu manunibilical cord blood hematopoietic cells were transplanted into SRD mice, the survival rate and the hematopoiesis reconstitu tion of bone marrow were assessed. The survival rate, in the mice transplanted and untransplanted, were 90% and 10% re spectively. Bone marrow and spleen of suriival mice showed human leukocytic antigen CD45_cells. Presence of multilineage en graftment, including myeloid and erythroid lineages, were found indicating that immuture human cells home to the mouse bone mar ruw. Conclusion: engrament of umbilical cord blood cells is very useful to reconstitute hematopoiesis of SRD casualties. As cord blood has many advanteges over bone marrow and pheraphal blood, it is important in rescuing radiation accidenlal casualties.
出处
《苏州医学院学报》
1997年第5期809-810,共2页
Acta Academiae Medicinae Suzhou
基金
核工业总公司科研基金
关键词
放射损伤
小鼠
脐血
造血细胞移植
radiation damage
cord blood
transplantation, mice
