摘要
[目的]寻找适合高黎贡山特点的放线菌分离方法，为新种的发现和筛选奠定基础。[方法]采集高黎贡山海拔1500m下7个土样，以K2Cr2O7为抑制剞，对高黎贡山土壤放线菌的分布情况进行了初步的研究。[结果]200mg／LK2Cr2O7具有很好的抑菌效果。放线菌种类丰富，但有机质丰富的土壤中放线菌的分布较耕地、旱地少。供试菌明胶液化培养5d后，中度液化能力的占42％。淀粉水解试验表明，绝大多数供试菌均有较强的水解能力，淀粉水解直径≤1cm的占13％，1～2cm的占40％，〉2cm的占47％。[结论]该研究为进一步对高黎贡山放线菌及微生物资源的研究提供了依据，也为高海拔微生物资源的调查研究做了方法上的准备。
[ Objective ] The aim Was to search for the actinomycete separation method suitable for the characteristics of Mt. Gaoligongshan and lay the foundation for the discovery and screen of new species. [ Method] 7 soil samples were collected from Mr. Gaoligongshan below 1 500 m altitude and with K2Cr2O7 as inhibitor, the distribution of soil actinomycetes from Mt. Gaoligongshan was studied preliminarily. [ Result] 200 mg/L K2Cr2O7 had good antibacterial effect. Actinomycete had abundant species, but its distribution in the soil with rich organic matter was less than that in plowland and dry land. After gelatin liquefaction of the tested actionmycetes for 5 d, the actionmycetes with moderate liquefaction ability was 42%. The starch hydrolysis test showed that the most tested actionmycetes all had stronger hydrolysis ability. The hydrolysis diameter of starch below lcm was 13%, that between 1 cm and 2 cm was 40% and that above 2 cm was 47%. [ Conclusion] The research provided the basis for the further study of actinomycetes from Mt. Gaoligongshan and microbial resources, and also made preparation for the survey method for the microbial resources in high altitude.
出处
《安徽农业科学》
CAS
北大核心
2008年第26期11456-11456,11464,共2页
Journal of Anhui Agricultural Sciences
关键词
高黎贡山
土壤放线菌
分布
水解
Mt. Gaoligongshan
Soil actinomycetes
Distribution
Hydrolysis
