摘要
目的:考察金银花总黄酮粗提物对临床主要致病菌的体外抑菌作用。方法:以DM130大孔吸附树脂为固定相,经不同浓度的乙醇梯度洗脱金银花水煎液得到各组分(水洗含糖较多组分为A、含糖较少组分为B,20%洗脱组分为C,40%洗脱组分为D,60%洗脱组分为E),测定各组分对临床主要致病菌的最低抑菌浓度(MIC)。结果:组分B对金黄色葡萄球菌有较强的抑制作用,MIC值为1mg·mL-1。结论:B组分对临床主要致病菌有较强的抑制作用。
OBJECTIVE： To study the in vitro antibacterial activity of the crude extract of total flavonoids from Lonicera japonica against the main pathogenic bacteria. METHODS： With DM130 macroporous resin as stationary phase, the different components （component A contained more sugar, and content B less sugar; C, D and E components were eluted by 20% ,40%, and 60% ethanol, respectively） were obtained by gradient elution of the aqueous extract from Lonicera japonica using different concentration of ethanol, then the MIC of different components on main pathogenic bacteria were detected.RESULTS： The antibacterial activity of component B against staphylococcus aureus was more ponent with its MIC at about 1 mg·mL^-1.CONCLUSION：Component B has ponent antibacterial action on main pathogenic bacteria.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第30期2321-2323,共3页
China Pharmacy
基金
国家自然科学基金资助项目(30572366)
关键词
金银花
总黄酮
MIC
Lonicera japonica
Total flavonoids
MIC
