摘要
目的:探讨Jagged1对树突状细胞(DC)分化的形态学影响。方法:应用骨髓法诱导培养DC,并利用原子力显微镜(AFM)观察细胞的超微结构。结果:与对照组相似,Jag-ged1组的细胞形态不规则,伪足分化和突出的片状结构较为明显,且表面粗糙;相反,DAPT组细胞的伪足不明显,多数细胞趋于圆形,表面相对光滑。此外,Jagged1组的细胞高于对照组和DAPT组,而直径则与对照组相当。结论:Jagged1对DC分化形态的影响不大,而DAPT组的细胞形态发生明显改变。
AIM：To explore the effect of Jagged1 on the morphology of dendritic cell（DC）differentiation.METHODS：Mouse bone marrow cells were cultured in the presence of rmIL-4 and rmGM-CSF.Atomic force microscopy（AFM）was used to observe the ultrastructure of these DC.RESULTS：Similar to the control,the cells in Jagged1 group displayed irregular shape with spiny or sheet-like dendrites and the cellular surface was rough.In contrast,the cells in DAPT group exhibited little dendritic processes and most of them were of round shape and with smooth surface.Furthermore,the height of the cells in Jagged1 group was higher than that in control and DAPT group,while the diameter of the cells in both was roughly equivalent.CONCLUSION：Jagged1 has no significant effect on the morphology of DC,but significant changes in the morphology of DC in DAPT group have occurred.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2008年第10期969-971,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30471635)
广东省自然科学基金资助项目(04010451
5006033)
