摘要
目的观察大鼠脑出血模型内源性神经干细胞（NSCs）的激活、增殖情况及其对神经行为学表现的影响．方法将72只SD大鼠按单双号分为脑出血组和假手术组，每组36只。脑出血组利用立体定向技术，将一定量的Ⅳ型胶原酶用微量进样器分别精确注入大鼠内囊诱导脑出血模型。假手术组注射等量体积的PBS。分别于术后1、7、14、21、28和35d观察大鼠的神经功能表现。所有大鼠处死前1d腹腔内注射5-溴脱氧尿嘧啶（BrdU）．免疫组织化学方法动态检测大鼠脑内巢蛋白（nestin）和BrdU的表达。结果假手术组大鼠脑内未见nestin和BrdU的表达。脑出血组血肿周围基底节和脑室下区可见nestin和BrdU的表达．脑出血后7d后开始明显增加，14d达高峰．21d开始下降，28d恢复正常。脑出血后1～35d大鼠神经功能无明显恢复，与内源性NSCs的增殖程度无明显相关。结论脑出血可导致内源性NSCs的激活和诱导其增殖；然而这种状态下NSCs的增殖能力和内源性NSCs对脑出血后神经功能缺损的修复均有限。
Objective To observe neural stem cell activation and proliferation in situ after intracerebal hemorrhage （ICH） and its effect on the neurological function of the injured adult rats. Methods Seventy-two adult rats were randomized into ICH and sham operation groups （n=36）. In the ICH group, type Ⅳ collagenase was injected into the internal capsule through a microinfusion pump to induce intracerebral hemorrhage, and the rats in the sham operation group received only phosphate buffer solution injection. The neurological functions of the rats were observed by rotarod motor test on days 1, 7, 14, 21, 28, and 35 after the injection. One day before sacrifice, the rats were subjected to intraperitoneal BrdU injection to label the regenerated cells, and immunohistochemistry was used to detect the expressions of nestin and BrdU in the brain tissue. Results No nestin- or BrdU-positive cells were found in the brain of the rats in the sham operation group. In rats with ICH, nestin- and BrdU-positive cells were found predominantly in the basal ganglion around the hematoma, in the ependyma and near the subventricular zone （SVZ） in the brain; the number of the positive cells increased significantly 7 days after ICH, peaked on day 14 and then significantly reduced on day 28. The rats exhibited no obvious improvement of the impaired motor function over the period from day 1 to 35 after ICH. Activation and proliferation of the neural stem cells was not obviously related to the recovery of the neurological functions. Conclusion Endogeneous neural stem cells in the brain are activated in rats after ICH, but these stem cells possess rather limited capacity of proliferation and can not sufficiently compensate for ICH-induced neurological function impairment.
出处
《中华神经医学杂志》
CAS
CSCD
2008年第10期997-1000,共4页
Chinese Journal of Neuromedicine
关键词
脑出血
神经干细胞
神经功能缺损
Intracerebral hemorrhage
Neural stem cells
Neurological function defect
